摘要
为了解决硝酸铝比色法在测定杜仲总黄酮时含量偏高的问题 ,用外标法和纸层析法对干扰测定的因素和测定方法进行了研究。结果表明 ,绿原酸是硝酸铝比色法测定杜仲黄酮的主要干扰因素 ,干扰程度大于5 0 % ;将纸色谱与硝酸铝比色法结合进行测定 ,可消除干扰 ,准确度提高 ,标准差为 10 .6 mg/ g,测定回收率为99.75 %。
It was found that when aluminum nitrate was used as a chromogenic agent in the colorimetric measurement of total flavonoids in the leaves of Eucommia ulmoides ,the result was deeply interfered by chlorogenic acid,a main chemical contained in the leaves,which possessed a structure of o phenolic di hydroxyls.Satisfactory result will be obtained if the paper chromatography is integrated with colorimetric measurement.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2001年第5期119-123,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省科技研究重大项目 ( 97K0 4-G2 )
关键词
杜仲叶
总黄酮测定
绿原酸
测定方法
Eucommia ulmoides leaf
measurement of flavonoids
chlorogenic acid
