摘要
本文应用细胞培养、免疫荧光及电镜技术研究了B淋巴母细胞对EHF病毒的易感性。结果表明EHF病毒可在该细胞中增殖。感染细胞无明显细胞病变,在形态上与对照组无差别。虽然大部分感染细胞呈现明亮病毒抗原荧光,但在电镜下却难以找到完整的病毒颗粒,仅在扩张的囊泡中发现一些性质待定的微丝样物质。人B淋巴母细胞持续感染的建立,提示患者外周血中大量出现的异型淋巴细胞可能允许EHF病毒在其中复制。
The susceptibility of human B lymphoblastoid cells to HFRS virus infectionwas studied. The results showed that the cells were productively infected byHFRS virus. The persistently infected cells had no obvious cytopathic effects andwere indistinguishable morphologically from uninfected cells. It was difficult toidentify complete virions in infected lymphoblasts by electron microscopy con-trasted with strong immunofluorescence staining for intracellular HFRS virusantigens observed in the majority of these cells. The establishment of persis-tently infected human B type lymphoblastoid cells suggests that the atypical ly-mphocytes with many characteristics of lymphoblasts regularly found in peri-pheral blood of HFRS patients may support replication of HFRS virus.
出处
《病毒学杂志》
CSCD
1989年第3期266-270,共5页
关键词
流行性出血热
病毒定位
B细胞
epidemic hemorrhagic fever virus
human B lymphoblasts
