摘要
目的：研究膀胱移行细胞癌中P16基因甲基化失活及其在肿瘤发生中的作用。方法：应用DNA甲基化分析技术检测52例膀胱移行细胞癌中P16基因5'CPG岛的甲基化状态，应用RT-PCR检测P16基因mRNA的表达。结果：52例膀胱移行细胞癌中19例出现P16基因5'CPG岛的甲基化，甲基化率为36.54%，而发生甲基化的肿瘤均未检测到P16mRNA表达。P16基因5'CPG岛甲基化多见于高期、高级肿瘤(P<0.05)。结论：P16基因5'CPG岛的甲基化引起的基因失活是膀胱肿瘤发展过程中的晚期所见。
objective: To investigate the role of p16 gene aberrant methylation in the malignant progression of human bladder cancer. Methods: p16 gene 5'CPG island methylation and mRNA expression of 52 cases bladder cancer were detected by DNA methylation analysis and RT-PCR respectively. Results: p16 gene 5'CPG island methylation was found in 19 of 52 (36.5%) cases of bladder cancer. There was no p16 gene mRNA expression in this tumor. p16 gene 5'CPG island methylation was significantly associated with tumor of higher grade and stage (P<0.05). Conclusion: p16 gene inactivation by 5'CPG island methylation might be a late event in the malignant progression of human bladder cancer.
出处
《伤残医学杂志》
2001年第1期24-27,共4页
Medical Journal of Trauma and Disability
