摘要
目的：观察电针“三阴交”对自然围绝经期大鼠的影响，探讨其对下丘脑-垂体-卵巢（HPO）轴紊乱的调节机制。方法：将3月龄SD雌性大鼠8只作为正常组，11～15月龄SD雌性大鼠阴道涂片法筛选符合围绝经期综合征表现者16只，随机分为模型组和电针组，各8只。电针组取两侧“三阴交”穴做电针，每次20min，隔日1次，共治疗15次。采用酶联免疫法测定各组大鼠下丘脑中促性腺激素释放激素（GnRH），垂体中卵泡刺激素（FSH）、促黄体生成素（LH）和卵巢中雌二醇（E2）的含量。结果：与正常组相比，模型组E2水平显著降低（P〈0．01），FSH、LH、GnRH水平均显著升高（P〈0．01）；与模型组相比，电针组E2水平明显升高（P〈0．05），FSH、LH和GnRH含量均有不同程度下降（P〈0．05）。结论：电针“三阴交”能够对围绝经期大鼠紊乱的HPO轴功能起到良性调节作用。
Objective To observe the effects of electroacupuncture （EA） at ＂Sanyinjiao＂（SP 6） on hypothalamus-pituitary-ovary （HPO） axis in perimenopausal rats so as to study its underlying mechanisms in improving dysfunction of HPO axis. Methods Eight female SD rats with an age of 3 months were used as the normal control group, other 16 perimenopausal SD rats with an age of 11 -- 15 months were equally randomized into model group and EA group. The menopause model was confirmed by vaginal smear tests for successive 15 days. EA （2 Hz/100 Hz） was applied to bilateral ＂Sanyinjiao＇（SP 6） for 20 min, once eve- ry other day for 30 days. After the treatment, the rats＇ hypothalamus, pituitary and left ovary tissues were collected for detecting the contents of gonadotropin releasing hormone （GnRH）, follicle-stimulating hormone （FSH）, luteinizing hormone （LH） and estradiol （E2） by ELISA. Results Compared with the normal control group, the ovary E2 content of the model group was significantly decreased （P〈0.01）, and the pituitary FSH and LH contents, and hypothalamic GnRH content were obviously increased in the model group （P〈0.01）. Following EA intervention, the ovary E2 level was obviously elevated （P〈0.05）, while pituitary FSH and LH and hypothalamic GnRH levels were significantly down-regulated in comparison with the model group （P〈0.05）. Conclusion EA of ＂Sanyinjiao＂ （SP 6） can suppress menopause-induced down-regulation of ovary E2 and increase of pituitary FSH and LH and hypothalamic GnRH levels in perimenopausal rats, suggesting a favorable regulation of EA on HPO axis dysfunction.
出处
《针刺研究》
CAS
CSCD
北大核心
2014年第3期198-201,共4页
Acupuncture Research
关键词
围绝经期综合征
电针
三阴交
下丘脑-垂体-卵巢轴
Perimenopausal syndrome
Electroacupuncture
Sanyinjiao （SP 6）
Hypothalamus-pituitary-ovary axis
