摘要
目的:探讨低氧对喉癌Hep-2细胞增殖及凋亡的影响。方法:模拟人体实体肿瘤低氧环境,应用MTT法检测低氧组与常氧组6、12、24、36h细胞增殖率,流式细胞学方法检测低氧组与常氧组6、12、24、36h细胞凋亡率。结果:常氧与低氧细胞增殖率随时间延长均呈逐渐升高趋势;常氧组与低氧组6、12、24、36h各时间点下比较于24h差异有统计学意义(P<0.01)。低氧组细胞凋亡率于6、12、24h较常氧组低(P<0.01),于36h较常氧组高(P<0.05)。结论:低氧可促进细胞增殖,且细胞增殖率可随时间延长逐渐增高。低氧能够抑制细胞凋亡,但持续缺氧还可以促进细胞凋亡。
Objective：To explore the effects of hypoxia on cell proliferation and apoptosis in human laryngeal cancer Hep-2 cells. Method：This experiment simulates the hypoxia environment of human solid tumor. Apoptosis rate and proliferation rate were detected by flow cytometry and MTT respectively. Both were measured under both the hypoxia and normoxia conditions at 6 h, 12 h, 24 h and 36 h, respectively. Result： The proliferation rate showed a trend of gradual increase under both hypoxia and normoxia conditions. The proliferation rate at 6 h, 12 h, and 36 h respectively were significant different from that at 24 h under both conditions （P〈0.01）. The apoptosis rate in hypoxic group were significant lower that in normoxic group at 6 h,12 h, 24 h, respectively （P〈 0.01）, while the opposite happened at 36 h （P〈0.05）. Conclusion： Hypoxia can promote cell proliferation, and cell proliferation rate can he increased gradually with time. Hypoxia can inhibit apoptosis, but persistent hypoxia may promote celt apoptosis.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2014年第12期871-873,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
低氧
增殖
凋亡
喉肿瘤
hypoxia
proliferatiom apoptosis
laryngeal neoplasms
