摘要
目的:探讨MUC1过表达与食管癌细胞对紫杉醇及顺铂反应的关系。方法:构建MUC1过表达的食管癌细胞株,建立食管癌细胞裸鼠移植瘤模型;然后,对成瘤裸鼠腹腔注射顺铂(8μg/kg,d1、d7)及紫杉醇(20μg/kg,d1、d7)。每3 d测量肿瘤体积及裸鼠体质量,绘制肿瘤生长曲线及裸鼠体质量曲线;测量肿瘤的终末体积,计算肿瘤抑瘤率;采用免疫组化方法检测化疗药物对肿瘤中Bcl-2及Ki-67表达的影响。结果:顺铂与紫杉醇都能抑制MUC1过表达的食管癌移植瘤的肿瘤体积及裸鼠体质量增加,与空载组相比差异有统计学意义(P<0.05),但顺铂与紫杉醇之间差异无统计学意义(P>0.05);顺铂和紫杉醇对MUC1沉默的食管癌移植瘤的抑制效应不明显;顺铂与紫杉醇能够明显下调MUC1过表达的移植瘤中Ki67的表达(P<0.01),上调Bcl-2的表达(P<0.01)。结论:顺铂与紫杉醇都能明显抑制MUC1过表达的食管癌移植瘤的生长及细胞增殖,促进细胞凋亡,顺铂的抑制效应与紫杉醇无明显差异,但顺铂对裸鼠体质量的影响较明显。
Objective：To investigate the relationship between MUC1 overexpression and response of esophageal cancer to pacli taxel and cisplatin. Methods： MUCl-overexpressed esophageal cancer cell lines were constructed and esophageal cancer cells xenograft models in nude mice were established. Cisplatin （8 μg/kg, dl, d7） and paclitaxel （20 μg/kg, dl, d7） were injected intraperitoneally. Tumor volumes and weights of nude mice were measured every three days, and tumor growth curves and body weight curves were drawn. The terminal tumor volumes were measured and tumor inhibition rates were calculated. Effect of the chemotherapeutic drugs on the expression of Bcl 2 and Ki-67 in tumor cells were detected by immunohistochemistry. Re- suits： Cisplatin and paclitaxel could suppress tumor growth of MUC1 overexpressed esophageal cancer. The weights of nude mice and tumor volumes in cisplatin group and paclitaxel group were less than those in no-load group, and the differences were statistically significant （P〈0. 05）, but no obvious difference was found between cisplatin group and paclitaxel group （P〉 0.05）. Meanwhile, significant inhibitory effect was not observed in stable MUCl-silenced esophageal cancer in nude mice. Cis- platin and paelitaxel could up-regulate the expression of Bcl 2 （P〈 0. 01）, but down regulate the expression of Ki-67 （P 〈0.01）. Conclusions： Cisplatin and paclitaxel can suppress the growth and proliferation but promote the apoptosis of MUC1 overexpressed esophageal cancer cells. The inhibitory effect has no obvious difference between cisplatin and paclitaxel （P〉 0.05）, but cisplatin can obviously affect the weights of nude mice.
出处
《中国临床医学》
2014年第1期1-4,共4页
Chinese Journal of Clinical Medicine
基金
上海市科学技术委员会基金项目资助(编号:11140903603)
关键词
食管癌
MUC1
紫杉醇
顺铂
Esophageal cancer
MUC1
Cisplatin
Paclitaxel
