摘要
以大豆粉为原料,分别以超声波辅助和微波辅助提取大豆异黄酮。在单因素实验基础上,通过正交试验优化确定最佳提取工艺。结果表明:大豆提取异黄酮工艺方法中超声辅助提取法优于微波辅助提取法,其最佳工艺条件为:乙醇质量分数80%,超声时间20 min,超声温度50℃,料液比1∶20。金属离子除K+和Ca2+外,Zn2+、Cu2+、Mg2+对大豆异黄酮抗氧化活性稳定性影响较为明显。这可为大豆异黄酮的综合利用开发提供依据。
The extraction conditions of soy isoflavones from soybean were optimized through orthogonal ex-periments on the base of single-factor tests. The results showed that the ultrasonic-assisted extraction method was better than microwave-assisted method, and the obtained optimum parameters for ultrasonic-assisted extraction method were as follows：concentration of ethanol 80%, ultrasonic time 20 min, ultrasonic temperature 50 ℃, ratio of liquid to material 20 mL/g. The stability of antioxidant activity of soybean isoflavones is significantly influenced by metal ions Zn2＋, Cu2＋and Mg2＋, but less influenced by K＋and Ca2＋metal ions. These results provide a basis for the application and comprehensive development of soybean isoflavones to some extent.
出处
《西部皮革》
2014年第6期16-21,共6页
West Leather
基金
陕西理工学院大学生科技爱好者协会--大学生创新实验项目(项目编号UIRP14001)
关键词
大豆
超声波
微波
大豆异黄酮
抗氧化稳定性
soybean
ultrasonic wave
microwave
soybean isoflavones
antioxidation stability
