摘要
采用电鳐胆碱酯酶抑制法测定大鼠血液中梭曼残留浓度 ,考察了丁酮肟 (DAM)及酰胺磷定 (HI 6)对大鼠血液中梭曼消除的影响 .结果表明 ,4 15μg·kg- 1梭曼 ( 5LD50 )iv后 3min ,DAM预处理有加速梭曼在大鼠血液中消除的趋势 ,使血中梭曼浓度由( 86± 2 2 ) μg·L- 1下降到 ( 53± 9) μg·L- 1,这可能与DAM加速了大鼠体内主要解毒器官中膦酰化羧酸酯酶 (CaE)的脱膦酰化速率有关 ,使有限的酶分子结合了更多的梭曼 .相比之下 ,HI 6预处理则对大鼠血液中梭曼浓度没有明显影响 .提示在大鼠体内 。
The effects of both carboxylesterase reactivator, diacetylmonoxime(DAM) and cholinesterase reactivator, HI 6 on the disappearance of soman from blood in rats intoxicated with soman(415 μg·kg -1 ,5 LD 50 ,iv) were studied using electric eel acetylcholinesterase based microassay. In rats pretreated with DAM the soman concentration was significantly reduced during 3-10 min, which might be partly due to its reactivation of carboxylesterase in detoxifying organs, and thereby increased efficacy of endogenous detoxifying enzymes. By contrast, HI 6 had negligible effects on soman clearance from blood in rats. It suggests that carboxylesterase is more important than cholinesterase in soman detoxification in rats.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2001年第1期65-67,共3页
Chinese Journal of Pharmacology and Toxicology
关键词
梭曼
羧酸酯酶
胆碱酯酶
丁酮肟
酰胺磷定
代谢解毒
soman
carboxylesterase
cholinesterase
reactivator
diacetylmonoxime
pyramidoxime
metabolic detoxication, drug
