摘要
目的探讨雷公藤甲素(triptolide)灌胃给予SpragueDawley(SD)大鼠4周后对肝脏血红素加氧酶(HO-1)的影响。方法 SD大鼠随机分成4组:对照组,雷公藤甲素(45、90、180μg·kg-1)组。灌胃给药,每天1次,连续4周。末次给药后隔天取材,采用实时定量PCR检测肝脏组织中HO-1基因的mRNA表达水平,Western blot检测肝脏中HO-1的表达量和NF-κB的转位入核情况。结果给予雷公藤甲素4周后,大鼠肝脏中HO-1表达增加,其中45μg·kg-1剂量组增加效果最为明显,约为4.5倍;NF-κB的核内水平随给药剂量增加而降低,180μg·kg-1剂量组NF-κB的降低至空白对照组的约1/3;NF-κB的核内水平随给药剂量增加而降低。结论重复给予雷公藤甲素可诱导大鼠肝脏中HO-1表达,抑制NF-κB的转位入核,从而对抗氧化应激和炎症反应。
Aim To investigate the effect of triptolide on heine oxygenase-I （HO-I） in Sprague-Dawley（SD） rats after gavage administration of triptolide for 4 week. Methods SD rats were randomly assigned into four groups. At the end of 4-weeks treatment, rats were sacrificed and liver tissues were collected for de-termination of levels of HO-1 mRNA expression by Re-al-time PCR and detection of HO-1 protein levels and NF-KB translocation by Western blot. Results Trip- tolide could increase the expression of HO-1 in livers of rats after administration for 4 weeks, while a dose of 45 μg . kg-1 caused a significant increase in the levels of HO-1 by about 4.5 fold. The expression of NF-KB inthe cell nucleus was also repressed with the increase of dose of triptolide. A dose of 180 μg . kg-1 caused a decrease of the expression of NF-KB by about one third compared with control group. Conclusion The results above suggest triptolide can play a role in response to anti-oxidant stress and anti-inflammation by inducing HO-1 and suppressing NF-KB nuclear translocation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第2期217-220,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81102886)
