摘要
目的分析2011年昆明市手足口病患儿粪便中分离获得的人鼻病毒（human rhinovirus,HRV）A22型分离株KM4/2011全长VP1基因的遗传特征。方法采用KMB17细胞对手足口病患儿粪便样本中筛查出的1株HRV样品进行病毒分离,应用RT-PCR法扩增病毒VP1基因,并进行测序,采用BLAST软件对所测定的节段序列进行数据库比对;采用Geneious basic 5.6.5软件进行核苷酸和氨基酸同源性比对;采用Mega 5.05软件将HRV A22及部分HRV A、B和C群全长VP1基因构建系统进化树。结果分离株为KM4/2011,经扩增、测序和序列拼接获得长度为867 bp的VP1基因;KM4/2011株与其他HRV分离株核苷酸和氨基酸的同源性分别为48.1%-91.0%和38.2%-97.6%,其中与HRV A22分离株的核苷酸和氨基酸同源性较高,分别为90.8%-91.0%和97.3%-97.6%;基因进化树分析显示,KM4/2011株与HRV A22基因型属于同一个进化分枝。结论 KM4/2011分离株为HRV A22型,存在地域差异。
Objective To analyze the genetic characters of VP1 gene of human rhinovirus A22 KM4 / 2010 strain isolated from the feces of children with hand-foot-mouth disease in Kunming City,Yunnan Province,China in 2011. Methods KM4 / 2011 strain was isolated by using KMB17 cells,from which VP1 gene was amplified by RT-PCR and sequenced,then compared with those of other rhinovirus strains in GenBank by using BLAST and Mega 5. 05 software. Results The VP1 gene at a length of 867 bp was obtained by amplification by RT-PCR,sequencing and splicing,of which the homologies of nucleotides and amino acids were 48. 1% - 91. 0% and 38. 2% - 97. 6% to those of other representative rhinovirus strains,and 90. 8% - 91. 0% and 97. 3% - 97. 6% to those of rhinovirus A22 isolate,respectively. Phylogenetic analysis revealed that KM4 / 2011 strain and other rhinovirus A22 strains were segregated into one distinct cluster. Conclusion KM4 / 2011 strain was human rhinovirus A22,while regional difference of human rhinovirus A22 strain was observed.
出处
《中国生物制品学杂志》
CAS
CSCD
2014年第2期177-179,184,共4页
Chinese Journal of Biologicals
基金
云南省应用基础研究面上项目(2013FZ136)
