摘要
设计了一种喷壁式毛细管电泳安培检测装置 .用原儿茶醛和原儿茶酚作为模型化合物考察了分离电压、电极与毛细管出口之间的距离对噪音和电极响应的影响 ,研究了清洗步骤对测定结果重视性的影响 .对该检测器的性能进行了测试 ,其分离效率可达 1.2× 10 5(原儿茶醛 )和1.1× 10 5(原儿茶酚 ) ,原儿茶醛和原儿茶酚检测限分别为 0 .2 5和 0 .50 μg/mL .
An electrochemical cell designed for the capillary zone electrophoresis with end-column wall-jet amperometric detection is reported. The three- electrodes system was used, with carbon-disc or copper-disc electrode as working electrode, Ag/AgCl electrode as reference electrode and platinum as auxiliary electrode. The distance between the working electrode and the capillary outlet on base-current and electrode respond were studied in detail. The investigation showed that good reproducibility could be obtained by using optimum wash-step. Application of this electrochemical cell for electrophoresis separation of the model compound protocatechuic aldehyde and protocatechuic acid has been studied, and high resolution and low detection limits have been obtained.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
2000年第6期85-88,共4页
Journal of Fuzhou University(Natural Science Edition)
基金
福州大学科技发展基金!(XKJYM 0 0 - 0 5 )的资助
关键词
毛细管电泳
安培检测
原儿茶醛
原儿茶酚
capillary electrophoresis
amperometric detection
protocatechuic aldehyde
protocatechuic acid
