摘要
目的 :探讨体外脂多糖 (LPS)刺激枯否细胞 (KC)分泌肿瘤坏死因子 -α (TNF -α)及内皮素 - 1(ET - 1)的作用。方法 :采用大鼠肝KC分离与培养技术 ,动态观察LPS刺激KC分泌TNF -α和ET - 1的作用。结果 :LPS具有明显活化KC作用 ,在一定LPS浓度范围内对KC分泌的TNF -α及ET - 1有促进作用。结论 :LPS可促进体外培养的KC分泌TNF -α及ET - 1。
AIM:To investigate LPS(lipopolysaccharide)stimulated cytokine secretion from normal rat kupffer cells in vitro. METHODS: Kupffer cells were isolated from wistar rats liver and cultured.Tumor necrosis factor -α (TNF-α) and endothelin-1(ET-1) secreted by LPS stimulated kupffer cells were detected. RESULTS: LPS had an stimulative effect on kupffer cell activity. LPS in definite concentrations promoted kupffer cell secretion.CONCLUSION: LPS promotes kupffer cell secretion, which may be associated with liver injury induced by LPS.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第12期1298-1300,共3页
Chinese Journal of Pathophysiology
