摘要
目的:探讨儿茶酚抑素(CST)治疗对慢性心力衰竭(CHF)大鼠室性心律失常(VA)的影响。方法:雄性SD大鼠51只,随机分为对照(CTL)组(n=17)和CHF组(n=34)。CHF组给予连续7 d注射异丙肾上腺素(ISO)(5 mg·kg-1·d-1,ip),CTL组则用生理盐水作为对照(1 mL·kg-1·d-1,ip),造模结束2周后,进一步将CHF组再随机分为未治疗组(n=17)和CHF治疗组(CST组)(n=17)。治疗组给予连续3周注射CST(2nmol·kg-1·d-1,ip),未治疗组注射0.9%生理盐水(1 mL·kg-1·d-1,ip)。动物完成药物注射后,在整体心脏Langendorff灌流条件下行离体电生理研究,分别记录和测量左室前游离壁(LAF)心外膜单相动作电位(MAP)和心室有效不应期(VERP);行程控增频电刺激以观察动作电位时程(APD)电交替(ALT);给予Burst快速电刺激进行VA的诱发。用酶解法分离获得LAF处单个心室肌细胞,采用全细胞膜片钳技术记录L型Ca2+通道电流(I Ca-L)。结果:与CTL组相比,未治疗组I Ca-L峰电流密度、90%单相动作电位时程(MAPD90)、VERP、诱发APD-ALT最大起搏周长(PCL max)中位数及VA诱发率均显著增大(均P<0.01);而CST组MAPD90和VERP均增大(P<0.01),其它指标差异无统计学意义(均P>0.05);与未治疗组相比,CST组I Ca-L峰电流密度、MAPD90、VERP、诱发APD-ALT的PCL max中位数(80 ms vs 100 ms)及VA诱发率均减小(均P<0.05),而VERP/MAPD90增大。结论:CST治疗可减少CHF大鼠VA诱发率,其机制可能与CST抑制CHF大鼠I Ca-L有关。
AIM： To determine the effects of catestatin （CST） on ventricular arrhythmia （VA） in isolated rat hearts with chronic heart failure （CHF）. METHODS： Fifty-one male rats were randomly divided into 2 groups： control （CTL） group （n--17） and CHF group （n = 34）, which were injected with 0.9% normal saline （1 mL. kg-1 ~ d-l, ip） and isoproterenol （ ISO, 5 mg ~ kg - l . d - 1, ip） for 7 d, respectively. The echocardiography was used to assess the cardiac functions 2 weeks after the end of modeling in both groups. The CHF rats were divided into non-treatment group （ n = 17） and CST treatment group （CST group, n =17）. The rats in CST group was given CST （2 nmol ~ kg-l ~ d-l, ip） for 3 weeks, while 0.9% normal saline （1 mL ~ kg-l ~ d-~, ip） was applied to the rats in non-treatment group. To all the whole Langendorff-perfused hearts, the monophasic action potential （MAP） and the ventricular effective refractory period （VERP） were recorded and measured in left anterior free wall （LAF）. The programmed electrical stimulation and burst pacing were used to induce action potential duration （APD） alternans （ALT） and VA in the LAF, respectively. The car- diac myocytes of LAF were enzymatically isolated and the technique of whole-cell patch clamp was used to record L-type Ca2 ＋ current （/Ca-L） RESULTS ： Compared with CTL group, the peak Ica-L density, 90% of MAP duration （ MAPDg0 ）, VERP, median of maximum pacing cycle length （PCLma-L） inducing APD-ALT and incidence of VA （83.33% vs 16. 67% ） were significantly increased in non-treatment group （ all P 〈 0.01 ）. Compared with non-treatment group, the peak 1C^-L density, MAPDgo, VERP, median of PCLm~ inducing APD-ALT and incidence of VA were significantly de- creased in CST group （ all P 〈 0.05 ）. CONCLUSION： Treatment with CST reduces the incidence of VA in CHF rats, which might be associated with the inhibition of Ica-L.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第12期2128-2132,共5页
Chinese Journal of Pathophysiology
关键词
电生理
心力衰竭
室性心律失常
儿茶酚抑素
Electrophysiology
Heart failure
Ventricular arrhythmia
Catestatin
