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施万细胞从兔变性坐骨神经中的分离、纯化和培养 被引量:1

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摘要 目的探讨可以在较短时间内获得大量较高纯度兔施万细胞的途径。方法取新西兰大白兔Wallerian变性的坐骨神经,用组织块反复种植培养法培养施万细胞,联合使用机械剥离、双30 min差速贴壁法、抗有丝分裂的抗癌药物阿糖胞苷分离纯化施万细胞。结果本实验方法获得的施万细胞的纯度达到90.5%。结论本实验方法可以在较短时间内获得大量纯度较高的施万细胞。
出处 《广东医学》 CAS CSCD 北大核心 2013年第20期3108-3110,共3页 Guangdong Medical Journal
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  • 1王世清,范志海,沈忆新.不同部位取材的雪旺细胞培养与纯化研究[J].中国修复重建外科杂志,2011,25(2):166-170. 被引量:12
  • 2朱浩,王文进,丁文龙.高效的雪旺细胞体外培养方法[J].解剖科学进展,2007,13(2):144-147. 被引量:13
  • 3WEI Y,GONG K, ZHENG Z, et al. Schwann - like cell differenti-ation of rat adipose - derived stem cells by indirect co - culture withSchwann cells in vitro[J]. Cell Prolif, 2010, 43(6) : 606 - 616.
  • 4SCARPINI E, KREIDER B Q, LISAK R P, et ai. Culture of hu-man Schwann cells isolated from fetal nerves [J]- Brain Res,1988, 440(2) : 261 -266.
  • 5SPIEGEL I, PELES E. A novel method for isolating Schwann cellsusing the extracellular domain of Necll [ J ]. J Neurosci Res,2009,87(15) : 3288 -3296.
  • 6NIAPOUR A, KARAMALI F,KARBALAIE K, et al. Novelmethod to obtain highly enriched cultures of adult rat Schwanncells[J]. Biotechnol Lett, 2010,32(6) ; 781 -786.
  • 7田林强,陈钢,杨彩虹,郭风劲,陈安民,陈少坚,孙凯.体外纯化乳鼠雪旺细胞的三种方法比较[J].实用医学杂志,2010,26(1):31-34. 被引量:5

二级参考文献38

  • 1杨俊,武雷,黄涛,余磊,邱小忠,廖华,陆云涛,刘晓静,秦建强.改良雪旺细胞传代培养的实验研究[J].中国临床解剖学杂志,2005,23(1):17-19. 被引量:16
  • 2翁惠兰,孙淑明,吴丽娥.雪旺细胞体外培养和纯化的新方法[J].中国基层医药,2005,12(10):1299-1300. 被引量:7
  • 3李锋,何晶,丁文龙.体外培养雪旺氏细胞纯化方法的探讨[J].解剖科学进展,2006,12(2):156-159. 被引量:11
  • 4何晶,李锋,丁文龙.雪旺氏细胞体外培养不同方法的比较[J].解剖学杂志,2006,29(3):384-386. 被引量:10
  • 5Saberi H, Moshayedi P, Aghayan H R, et al. Treatment of chronic thoracic spinal cord injury patients with autologous Schwann cell transplantation: An interim report on safety considerations and possible outcomes [J]. Neurosci Let ,2008, 443( 1 ) :46-50.
  • 6Mauritz C, Grothe C, Haastert K. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Sehwann cells [J]. J Neurosei Res,2004, 77 (3) :453-461.
  • 7Pannunzio M E, Jou I, Long A, et al. A new method of selecting Schwann cells from adult mouse sciatic nerve [J]. J Neurosei Meth, 2005,149( 1 ) : 74-81.
  • 8Jirsova K,Sodaar P,Mandys V, et al. Cold Jet : A method to obtain pure Schwann cell cultures without the need for cytotoxic, apoptosisinducing drug treatment [J]. J Neurosci Meth, 1997,78(1-2) : 133- 137.
  • 9Vroemen M, Weidner N. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Meth, 2003, 142(2) : 135- 143.
  • 10Magnaghi V, Veiga S, Ballabio M, et al. Sex-dimorphic effects of progesterone and its reduced metabolites on gene expression of myelin proteins by rat Schwann cells [J]. J Periph Nerv Sys,2006, 11(2):111-118.

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