摘要
以平板双分子层脂膜作为生物膜的简单模型 ,建立用平板双分子层脂膜电性质研究药物 -生物膜相互作用的方法。研究以具有典型特征的物质 -表面活性剂、自由基、金属手性配合物与平板膜的相互作用引起膜电性质的规律性改变 ;重组人B型血红细胞膜与溶液中抗B单克隆抗体发生特异相互作用时 ,膜电阻快速下降 ,下降的速率与加入的抗体量成正相关。在研究发生在平板膜上的典型反应的基础上 ,通过对膜电性质的监测和分析 ,从而确认平板双分子层脂膜能够成为研究药物 -生物膜相互作用和药物跨膜渗透的有力工具。
Drug-biomembrane interaction was studied by measuring the electrical properties of bilayer lipid membranes(BLM) as a simple model of biomembranes. The typical substances-surfactants, radical and metallic chiral complexes caused regular changes of membrane electrical parameters. The specific interaction between the reassembled type-B human erythrocyte membranes and its ligand, anti-B monoclonal antibody, caused a rapid decrease in membrane resistance(Rm) and membrane breakdown finally. The magnitude of Rm decrease is positively related to the amount of antibody added. This suggests that BLM can be a useful instrument to study drug-membrane interaction and drug permeation, by measuring and analyzing its electrical parameters.
出处
《生物物理学报》
CAS
CSCD
北大核心
2000年第4期694-700,共7页
Acta Biophysica Sinica
基金
国家自然科学基金资助项目(39770879)
