摘要
目的探讨白花蛇舌草乙醇提取物对小鼠结直肠癌血管生成的抑制作用.方法构建32只BALB/c小鼠皮下CT26结肠癌动物模型,随机分为4组,每组8只小鼠,分别为Ⅰ组:对照组生理盐水0.1 mL/(10 g.d),Ⅱ组:白花蛇舌草乙醇提取物90 mg/(kg.d),Ⅲ组:白花蛇舌草乙醇提取物180 mg/(kg.d),Ⅳ组:白花蛇舌草乙醇提取物360 mg/(kg.d).各组小鼠在接种CT-26结肠癌细胞后的第10天开始测量肿瘤的长径(mm)和短径(mm);接种后第12天开始采用灌胃给药法给药,连续给药10 d后停止给药,继续喂养观察至第32 d后处死小鼠,取肿瘤组织用免疫组化法检测微血管密度.结果Ⅱ组、Ⅲ组和Ⅳ组小鼠的肿瘤体积明显小于Ⅰ组,差异有统计学意义(P<0.05);Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组的肿瘤组织微血管密度分别为(7.83±2.87)、(5.32±1.27)、(1.77±0.70)、(1.87±0.68);Ⅱ组、Ⅲ组和Ⅳ组肿瘤组织血管数量明显少于Ⅰ组,差异有统计学意义(P<0.05).结论在一定剂量范围内,白花蛇舌草乙醇提取物对小鼠CT-26结肠癌有明显的抑瘤作用,其机理可能是通过抑制小鼠结肠癌血管生成而起到抗肿瘤的作用.
Objective To explore the inhibitory effect of Oldenlandia diffusa extract on colorectal cancer angiogenesis in BALB/c mice. Methods Thirty-two BALB/c mice with subcutaneous CT26 colon cancer animal model were randomly equally divided into four groups,including the control group （groupⅠ,saline 0.1 mL/（10. d）, O. diffusa ethanol extract of 90 mg/（kg.d） （groupⅡ）, O. diffusa ethanol extract of 180 mg/（kg.d） （groupⅢ） and O. diffusa ethanol extract of 360 mg/（kg.d） （group Ⅳ） . Each group of mice were treated with intragastric administration of law administration 12 days after vaccination, then stopped and continue fed to 32 days, and the mice were killed. Micro-vascular dense （ MVD） was observed and countered under the microscopy by immunohistory chemistry. Results The murine colon tumor volumes of GroupⅡ,ⅢandⅣwere significantly less than that of groupⅠ,with significant difference （ 〈0.05） . The tumor microvessel density values of four groups was （7.83±2.87）, （5.32±1.27）, （1.77±0.70） and （1.87±0.68）,respectively. The number of tumor blood vessels in GroupⅡ,Ⅲ and Ⅳ were significantly less than that of Ⅰ group, with significant difference （ 〈0.05） . Conclusion Within a certain dose range, the ethanol extract of O. diffusa can significantly inhibit the mouse colon cancer and the mechanism may be realated to inhibiting tumor angiogenesis.
出处
《昆明医科大学学报》
CAS
2013年第10期53-57,共5页
Journal of Kunming Medical University
基金
云南省卫生厅科技计划项目(2011WS0065)
