摘要
目的观察超深低温(-196℃)条件下不同冷冻时间对异体神经移植后生理特性的影响。方法将80只雌性Wis tar大鼠随机分为取材组(20只),对照组(A组:新鲜自体神经移植组;B组:新鲜异体神经移植组,每组10只)和实验组(按取材神经冷冻保存3、6、9、12周后移植分为C、D、E、F组,每组10只)。术后做大体、光镜、电镜观察,神经电生理测试。结果移植9周后,大体、光镜、电镜结果显示:B组生理特性影响最大,A组最小,C、D、E、F组次之;电生理结果显示:A与B、A与E、A与F之间差异有统计学意义(P<0.05)。结论异体神经移植后的生理特性和冷冻时间存在依从关系。
Objective To observe the effect of different conserve time in ultra deep cryopreservation （-196 ℃）on physiological characteristics of allograft nerve transplantation .Methods A total of 80 female Wistar rats were divide into three groups by random principle：derived group which 20 rats were sacrificed to get both sides of femoral nerves ;control groups which each group had 10 rats include group A for fresh autologous nerve transplantation ,group B for fresh allograft nerve transplantation ;experiment groups which each group had 10 rats including groups C ,D ,E ,F that transplanted after femoral nerves conserve in the -196 ℃ for 3 ,6 ,9 , 12 weeks respectively ,and the results of exterior appearance ,light microscope ,electron microscope were observed and electrophysi-ological test was conducted after transplantation .Results After 9 weeks transplantation ：the physiological characteristics of group B was most affected ,followed by group C ,D ,E ,F ,group A was with minimal impact ;the result of electrophysiological test showed that groups A＆B ,A＆E ,A＆F had significant difference（P〈0 .05） .Conclusion The physiological characteristics of allograft nerve transplantation relate to the freezing time .
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第29期3521-3523,3526,共4页
Chongqing medicine
关键词
低温保存
异体神经
冷冻
移植
生理特性
大鼠
WISTAR
cryopreservation
allograft nerveifreezingltransplantation
physiological characteristics
rate,Wistar
