摘要
为研究母源性印记基因Magel2、Sgce、SNRPN在牛早期胚胎中的表达规律,初步判定其印记发生的阶段。利用Real-time PCR技术,检测了这3个基因在牛孤雌激活（Parthenogenetic Activation,PA）胚胎和体外受精（In vitro Fertilization,IVF）胚胎中的表达水平。结果表明：在牛IVF和PA 2种胚胎间,3个母源性印记基因的印记表达模式明显不同,Magel2在牛IVF胚胎各阶段都有表达,而在PA胚胎中没有检测到表达信号;Sgce在牛IVF胚胎各阶段都有表达,而只在PA胚胎的2~4细胞阶段检测到表达,在其他发育阶段均没有表达;SNRPN在牛IVF胚胎各阶段都有表达,而只在PA胚胎的2细胞和囊胚阶段检测到很微弱的表达。实验结果表明,母源性印记基因Magel2、Sgce、SNRPN的正常表达对牛早期胚胎发育具有重要作用。
In order to detect the expression profiles of the maternal imprinted genes Magel2, Sgce and SNRPN in the earlier bovine embryos, as well as preliminary determine the stage from which the imprinting start. In this study, the expression profiles of three maternal imprinted genes were detected between bovine Parthenogenetic Activation （PA） and in vitro Fertilization （IVF） embryos by Real-time PCR. The results showed that the expression patterns of the three genes were significantly different between IVF and PA embryos. Magel2 was expressed from 2 to blastocyst in bovihe IVF embryos, while no detectable transcripts were observed in PA embryos. Sgce was expressed from 2 to blastocyst in bovine IVF embryos, while 2 to 4 cell stage in PA embryos. In bovine IVF embryos SNRPN transcripts could be detected from 2 to blastocyst stage, but it was only detected in 2-4 cell stage PA embryo. SNRPN expression could be detected in bovine IVF embryos from 2 cell to blastocyst stages, meanwhile it was only detected in PA 2 cell and blastocyst stage
出处
《中国农学通报》
CSCD
2013年第26期9-13,共5页
Chinese Agricultural Science Bulletin
基金
国家科技计划项目"家畜胚胎保存与性控双犊诱导技术研究与示范"(2011BAD19B01)
内蒙古自然科学基金资助项目"印记基因对牛胚胎发育影响的研究"(2010MS0407)
关键词
牛
母源印记基因
早期胚胎
表达
bovine
maternal imprinted gene
early stage embryo
expression
