摘要
建立了快速检测食源性金黄色葡萄球菌(SA)16SrDNA、纤维蛋白结合蛋白(clfa A和clfa B)、纤连蛋白结合蛋白(fnbp A和fnbp B)的多重PCR方法.利用GenBank SA的16SrDNA,clfa A,clfa B,fnbp A和fnbp B基因序列,设计5对特异性引物,建立鉴定SA及分析SA毒素基因的五重PCR方法,并对160株食源SA进行属鉴定和毒素基因检测.结果显示,供试的160株食源SA中葡萄球菌属16SrDNA鉴定均为阳性;4种SA毒素基因检出率由高到低依次为clfa A(91.88%),fnbp A(21.88%),clfa B(19.38%),fnbp B(8.13%).该方法简便、快捷、准确,为食源SA的属鉴定和毒素基因分析提供了快速检测方法,同时可作为食源性疾病事件处理的溯源技术.
According to gene sequences of 16S rDNA, clfa A,clfa B, fnbp A and fnbp B of Staphylococcus aureus （SA） in GenBank, five pairs of specific primers were designed and used in multiplex PCR for identification and detection of SA toxin genes. Multiplex PCR was developed and optimized in the detection of 160 food-borne SA isolates. The positive rates for detection of 16S rDNA,clfa A,clfa ]3,fnbp A and fnbp B genes by PCR were 100 %, 91.88 %, 19.38 %,21.88 % and 8. 13%, respectively. The results indicated that multiplex PCR assay was convenient,timesaving and accurate. Our study has provided a rapid detection method for identification of food-borne SA,molecular analysis of toxins in SA,and the investigation of the origin of food-borne diseases.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2013年第5期514-518,共5页
Journal of Hebei Normal University：Natural Science
基金
石家庄市科学技术研究与发展指导计划(111461683)
