摘要
目的探讨参黄胶囊对环磷酰胺致免疫抑制模型小鼠免疫功能的作用。方法分别用环磷酰胺和限食法结合环磷酰胺两种方法建立小鼠免疫抑制模型,分别给予0.26、0.53、1.06g/kg参黄胶囊,观察其对小鼠外周血T淋巴细胞率、胸腺指数、脾脏指数、白细胞数、巨噬细胞吞噬指数、血清溶血素含量(半数溶血值)的影响。结果应用环磷酰胺后,免疫抑制模型小鼠外周血T淋巴细胞率、胸腺指数、脾脏指数、白细胞数、巨噬细胞吞噬指数、血清溶血素含量均显著降低。0.26、0.53、1.06g/kg参黄胶囊均可明显提高用限食法结合环磷酰胺致免疫低下(气虚模型)小鼠外周血T淋巴细胞率、胸腺指数和脾脏指数和环磷酰胺致免疫低下小鼠体内白细胞数和巨噬细胞吞噬指数;0.53、1.06g/kg参黄胶囊可明显促使溶血素生成增加。结论参黄胶囊可明显改善环磷酰胺致免疫低下小鼠的免疫功能,具有扶正固本、增强机体特异性免疫和非特异性免疫功能作用。
Objective To explore the effects of Shenhuang Capsule on immunofunction of cyclophosphamide （CY）- immunodepressed mice. Methods The immunosuppressive mouse models were established respectively by CY and CY combined with food restriction. After ig administration of Shenhuang Capsule （0.26, 0.53, and 1.06 g/kg）, the effects of Shenhuang Capsule on T lymphocyte percentage in peripheral blood, thymus index, spleen index, leucocyte count, macrophage engulfing index, and serum haemolysin content （HC 50 ） in mice were observed. Results There were significant reductions in T lymphocyte percentage in peripheral blood, thymus index, spleen index, leucocyte count, macrophage engulfing index, and HC 50 in immunodepressive mice model induced by CY. Shenhuang Capsule （0.26, 0.53, and 1.06 g/kg） could obviously improve T lymphocyte percentage in peripheral blood, thymus index, and spleen index in immunosuppressive （Qi-asthenia model） mice treated by CY combined with food restriction, and elevate the leucocytes and macrophage engulfing index in CY-immunodepressed mice. Shenhuang Capsule （0.53 and 1.06 g/kg） could significantly promote haemolysin formation in CY-immunodepressed mice. Conclusion Shenhuang Capsule could significantly improve the immunofunction of CY-immunodepressed mice and have the effects on fostering resistance to disease and enhancing the body’s specific and nonspecific immunofunction.
出处
《现代药物与临床》
CAS
2013年第4期504-507,共4页
Drugs & Clinic
关键词
参黄胶囊
环磷酰胺
小鼠免疫抑制模型
免疫功能调节
Shenhuang Capsule
cyclophosphamide
immunosuppressed mouse model
immunofunction regulation
