摘要
为研究草鱼头肾的组织发生发育,应用组织学技术,对1～103 dph(day post hatching)的草鱼头肾进行观察分析,描述了头肾的组织结构变化及免疫球蛋白M(IgM)阳性细胞及过碘酸雪夫氏(PAS)阳性细胞的发生和分布。结果显示,6 dph,出现头肾原基,原基中原肾管分化形成的肾小管之间出现散在的干细胞。7 dph至16 dph,造血干细胞逐渐分化成不同类型的细胞,免疫细胞数量逐渐增加;17 dph至103 dph,肾小管逐渐退化至完全消失,网状内皮系统支持下的淋巴造血组织构成头肾的主要部分。40 dph肾上腺细胞团开始出现。65 dph与成鱼头肾的组织结构接近,作为淋巴—肾上腺组织存在。PAS阳性细胞最早在7 dph出现;IgM阳性细胞最早出现于17 dph;之后,IgM阳性细胞及PAS阳性细胞逐渐增多,分布于整个头肾组织。研究表明,65 dph草鱼头肾在组织上发育基本成熟;头肾作为草鱼免疫器官的早期发育可以分为3个阶段:无参与免疫反应的细胞、只具有参与非特异性免疫反应的细胞以及参与非特异性免疫反应及特异性免疫反应的细胞组织结构逐步发育完善。
Histogenesis of the head kidney was examined in the collected grass carp（Ctenopharyngodon idella）from 1 to 103 days post hatching（dph）.Histological technology was applied to learn the change of the structure of the head kidney and the changes in the distribution of IgM（immunoglobulin M）positive cells and PAS（periodic acid schiff reaction）positive cells in head kidney.The primordium of the head kidney formed at 6 dph.And in the primordium several undifferentiated hemopoietic stem cell spread among the renal tubules which were derived from the protonephridia.Then,the immune cells increased gradually due to the hemopoietic stem cells＇ differentiation from 7 dph to 16 dph.Between 17 dph and 103 dph,the renal tubules degenerated gradually and the lymph and haematogenous tissues sustained by the reticulo-endothelial system（RES）became the major component of the head kidney.By 65 dph,the head kidney was histologically similar to mature fishes,formed as a lymphoid-adrenergic tissue.Cells producing IgM as lymphocytes were detected by immunohistochemistry（IHC）stain.A few PAS positive cells were observed by 7 dph while several IgM positive cells were occasionally first detected at 17 dph.PAS positive cells and IgM positive cells were both increased with age and finally scattered all over the head kidney.In short,the head kidney in grass carp was almost mature histologically by 65 dph;as an immune organ,the development of the head-kidney in juvenile grass carp can be divided into three stages characterized by the Ⅰ-absence of cells involved in immunity,Ⅱ-presence of cells only involved in nonspecific immunity,and Ⅲ-emergence and proliferation of cells involving in both nonspecific and specific immunity.
出处
《水产学报》
CAS
CSCD
北大核心
2013年第6期840-850,共11页
Journal of Fisheries of China
基金
国家科技支撑计划(2012BAD25B00)
现代农业产业技术体系建设专项(CARS-46)
关键词
草鱼
头肾
组织发生
免疫组织化学
PAS阳性细胞
Ctenopharyngodon idella
head kidney
histogenesis
immunohistochemistry
PAS positive cells
