摘要
采用胰酶消化法建立了大鼠黄体细胞体外分离方法。在整个操作过程中 ,黄体细胞存活率均在95 %以上。研究结果表明 :孕期 14～ 2 0天大鼠、黄体细胞密度 10 6个 / ml、培养时间 4小时是适宜的体外分离培养条件。在对某市自来水厂氯化饮用水有机浓集物 (每孔相当于 5 0 0、10 0 0、30 0 0、5 0 0 0 ml自来水 )进行毒性测试时发现 :各试验浓度组均可使黄体细胞孕酮分泌量显著增加 (P<0 .0 1) ,呈现出明显的剂量 -效应关系。故认为氯化饮用水有机浓集物可导致大鼠黄体细胞孕酮分泌量显著增加 ,从而影响黄体细胞的正常生理功能。
A separation method of rat luteal cells was established. The results show that the survival rate of the cells is more than 95% during the whole operation. 14～20 days of pregnancy,10 6 cells/ml and 4 hours of culture are the suitable conditions. The organic extracts of drinking water produced significant dose related increases(maximally three fold) in progesterone concentration in the culture medium. Thus, the effects produced by the organic extracts of drinking water in vitro may reflect the damage to the luteal cells.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第3期143-144,共2页
Journal of Hygiene Research
