摘要
目的:观察TLR4在RAW264.7细胞中的表达及其生物学效应。方法:不同浓度的LPS刺激RAW264.7细胞后,应用RT-PCR、细胞迁移实验和Wester blot检测细胞中TLR4表达以及对破骨细胞迁移率、破骨细胞内基质金属蛋白酶(MMP9)、酒石酸酸性磷酸酶(TRAP)、组织蛋白酶K(cathinK)的活性。结果:TLR4在RAW264.7中表达并且能被LPS激活,TLR4被激活后其mRNA及蛋白表达水平均增高,细胞内MMP9、TRAP、cathinK mRNA的表达水平和细胞迁移率均增高。结论:LPS可使RAW264.7中TLR4被激活,TLR4的激活可作用于RAW264.7而增高细胞内相关酶的活性及细胞迁移率。
Objective： To study the expression of Toll like receptor 4 （TLR4） in RAW 264.7 cells and its biological effects on the cells. Methods： RAW264.7 cells were stimulated with different concentrations of LPS, cell migration assay, RT-PCR and Western blot were used to study the migration of the cells, the mRNA and protein expression of TLR4 and mRNA expression of MMP9 ,TRAP and cathinK in RAW264.7 cells respectively. Results： TLR4 was expressed in RAW264.7 cells, the mRNA and protein expression levels were increased by LPS in a dose-dependant manner. The cell migration rate was increased and the mRNA expression levels of MMP9, TRAP and cathinK were increased by LPS treatment. Conclusion ： LPS may activate TLR4 in RAW264.7 cells. The activation of TLR4 can increase the migration rate and the related enzyme mRNA level in RAW264.7 cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2013年第3期303-307,共5页
Journal of Practical Stomatology
基金
国家自然科学基金资助项目(编号:31271030)
