摘要
目的：了解长期大脑中动脉闭塞（ＭＣＡＯ）后丘脑体积的改变。方法：３５只大鼠分７组，除正常组和伪手术组外．其余各组用Ｔａｍｕｒａ法闭塞大脑中动脉，用大鼠脑单克隆抗体微管蛋白－２（ＭＡＰ－２）对各组大脑切片进行免疫组化染色．计算机测量各组大鼠脑切片的丘脑面积。结果：正常组０．９９±０．０１，伪手术对照２周组１．０２±０．０２，伪手术对照１个月组０．９９±０．０１，ＭＣＡＯ２周组０．８７±０．０３，１个月组０．７８±０．０３，ＭＣＡＯ３个月组０．７１±０．０４，６个月组０．６１±０．０５，伪手术组和ＭＣＡＯ组之间在丘脑萎缩方而有显著差异（Ｐ＜０．００１）。结论：提示逆行性退行性变在丘脑萎缩方而起重要作用。
Aim: To investigate the change of thalamus after long-term permanent middle cerebral artery occlusion (MCAO). Methods: 35 rats were divided into 7 groups in average, the middle cerebral artery was occluded with Tamura method except normal and sham groups. All rat slices were stained by the rat brain monoclonal antibody MAP-2 and the areas of the right and left thalamus were measured by computer digitizer. Results: The ration of the ipsilateral and contralateral tha- lamus was 0.99±0. 01 at normal group, 1.0±0.01 at sham group, 0.87±0. 03 at MCAO 2-week group, 0.78±0.03 at MCAO 1-month group, 0.71±0.04 at 3-month group, 0.61±0. 05 at 6-month group. There was a significant difference be- tween the sham group and MCAO groups (P<0.001). Conclusion: It is indicated that retrograde degeneration is thought to play an important role in thalamus atrophy.
出处
《中国临床神经科学》
2000年第3期175-177,共3页
Chinese Journal of Clinical Neurosciences
