摘要
目的探讨中药制剂糖脂平颗粒对2型糖尿病大鼠血清胰岛素样生长因子-I（IGF-I）的影响。方法SPF级Wistar大鼠40只，雌雄各半，体重（200±20）g，基础饲料适应性饲养1周后，将大鼠按体重编号，以随机数字表法抽取8只为空白组，普通饲料喂养；其余大鼠均喂以高脂、高糖饲料。第6周，一次性左侧下腹部注射小剂量链脲佐菌素（STZ）诱导糖尿病模型。第8周，选择造模成功的30只大鼠称重并按体重编号，按照随机数字表法分为糖尿病模型组、糖脉康颗粒组、糖脂平颗粒组，分别予以生理盐水、糖脉康颗粒、糖脂平颗粒灌胃治疗。第12周处死大鼠，取血清检测各组大鼠血糖、血清IGF-I。结果①与空白组比较，糖尿病模型组血糖（28．33±0．50）mmol／L升高，IGF-I（3．40±0．83）pg／ml降低，说明造模成功。②与糖尿病模型组比较，糖脂平颗粒组、糖脉康颗粒组血糖[（15．06±0．39）mmol／L、（20．12±0．58）mmol／L]降低，IGF-I[（9．39±2．91）pg／ml、（5．58±1．09）pg／m1]升高，两组比较差异有统计学意义。③与糖脉康颗粒组比较，糖脂平颗粒组的血糖降低、IGF-I升高，差异均有统计学意义（P均〈0．05）。结论糖脂平颗粒可降低2型糖尿病模型大鼠血糖、升高IGF-I，改善糖尿病的症状。
Objective To explore the effects of Tangzhiping granules on the blood glucose and IGF- I in T2DM rats. Methods 40 SPF grade Wistar rats whose weight were about （200 ± 20） g were selected as the subjects for this study, one half were male and another half were female. After be adapted to the normal food for 1 week, 8 rats were selected randomly from all of rats following their weight as the blank group （ordinary feed） .The remaining the others were fed with the high-fat and high-sugar food. The 6th week, the other rats would be undergo intraperitoneal injection once in left lower belly with 40 mg/kg streptozotocin （STZ） , and enable them to become T2DM rats. The 8th week, we would select successful animal models 32 which were divided randomly into a modle group, a Tangmaikang （TMK） granules group, a Tangzhiping granules （TZP） group. The 12th week, we would execute all of rats and collect the blood serums which were tested for the blood glucose and IGF- I. Results ① Compared model group with blank group, the levels of the blood glucose of the model group increased to （28.33 ± 0.50）mmol/L, while the IGF- I decreased to （3.40 ± 0.83）pg/ml, which showed the model of type 2 diabetic rats was successfully set up. ② The levels of the blood glucose of the Tangzhiping granules group and Tangmaikang granules group were lower than that of the model group to （15.06±0.39）mmol/L, （20.12±0.58）mmol/L, but the levels of the IGF- I were higher to （9.39±2.91）pg/ml, （5.58 ±1.09）pg/ml, which implied that each group of pharmacological treatment had a good effect. ③ The level of the blood glucose and IGF- I of the Tangzhiping granules group was also significantly different from that of the Tangmaikang granule group, the therapeutic effect of Tangzhiping granules group was better than that of Tangmaikang granules group. Conclusion Tangzhiping granules can reduce the blood glucose of T2DM rats.And also can increase IGF-I of T2DM rats. Tangzhiping granules can improve the various symptoms of diabetes mellitus at the same time.
出处
《国际中医中药杂志》
2013年第4期326-328,共3页
International Journal of Traditional Chinese Medicine
