摘要
目的建立化生平合剂中黄芪甲苷的含量测定方法。方法采用高效液相色谱法,KromasilC18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水-磷酸(1:2:0.15),检测波长为203nm,流速为1.0mL.min-1,柱温为室温。结果黄芪甲苷在进样量0.5-10μg范围内线性关系良好,r=0.999 6;平均回收率为97.82%,RSD为1.79%。结论测定结果准确、可靠,有良好的精密度和重现性,可用于化生平合剂中黄芪甲苷的含量测定。
Objective To establish the method for determination of astragalosidelV in Hua Sheng Ping composition. Method The contents of astragalosidelV in Hua Sheng Ping composition were determined by HPLC. Kromasil C 18 column （4.6 mm250 mm, 5 μm） was used with acetonitrile-water-phosphoric acid （1：2：0.15） as mobile phase, and the detective wavelength was 203nm. The flow rate was 1.0 mL-min-1 and the column temperature was room temperature. Results AstragalosidelV had a good linear relation in the range of 0.5-10μg （r=0.999 6） and the average recovery was 97.82%, RSD was 1.79%. Conclusion The method has good precision and reproducibility, and the results were reliable and accurate. It could be used to determine the contents of astragalosidetV in Hua Sheng Ping composition.
出处
《现代中药研究与实践》
CAS
2013年第1期62-65,共4页
Research and Practice on Chinese Medicines
