摘要
采用分子标记、化学方法、酶标仪、统计学等方法,对99份粳稻新品种(系)进行Wx-mq基因(低直链淀粉含量的暗胚乳突变基因)检测,提取、测定各品种(系)直链淀粉含量,并分析不同遗传背景及不同生态类型材料间直链淀粉含量的差异,旨在为低直链淀粉含量的优良食味粳稻新品种选育提供参考依据。试验结果表明:99份材料均含有Wx-mq基因;供试材料的直链淀粉含量主要分布在8%与11%之间;武香粳14与关东194杂交育成的品种(系)直链淀粉含量的变幅相对较小;不同熟期品种(系)的直链淀粉含量的变幅为中粳较晚粳大。
The aim was to offer some recommendations for breeding new japonica rice varieties with good eating quality carrying low amylose content.Marker-assisted selection,chemical extraction,microplate reader and statistical methods were used to detect Wx-mq gene in 99 new rice varieties（lines） and analyze the amylose contents of materials with different genetic backgrounds and ecological types.Wx-mq gene was carried in 99 new rice varieties（lines）.The amylose contents were between 8% and 11%.The amylose contents of rice varieties（lines） derived from the combination of Wuxiangjing 14/Kanto 194 were relatively high,and the amplitude was relatively small.The amylose content differed among tested varieties（lines） with the order of medium-maturing medium japonica rice greater than medium-maturing late japonica rice,late japonica rice taking the least.The amplitude of variation of amylose content in medium japonica rice was bigger than that in late japonica rice.
出处
《江苏农业学报》
CSCD
北大核心
2012年第6期1218-1222,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(10)306]
江苏省产学研前瞻性联合研究项目(BY2010134)
江苏省重大科技支撑计划项目(BE2011302)
关键词
水稻
暗胚乳突变基因
直链淀粉含量
食味品质
rice（Oryza sativa L.）
translucent endosperm mutant gene
amylose content
eating quality
