摘要
选择初始体重(3.27±0.04)g的吉富罗非鱼(GIFT Oreochromis niloticus),分别投喂基础饲料和添加80、160、240、320和400 mg/kg谷胱甘肽(GSH)的试验饲料,记作G0、G80、G160、G240、G320和G400。49 d后,G320血清和G160、G240肝脏谷胱甘肽S-转移酶,G160、G240血清和G320肝脏谷胱甘肽还原酶,G240肝脏超氧化物歧化酶,G320血清和G240肝脏过氧化氢酶活性,G240～G400血清和G240肝脏总抗氧化能力,G320血清抗超氧阴离子活性,与对照组相比分别显著升高。G80～G320肝脏丙二醛含量显著低于对照组。在亚硝基氮应激96 h内,G320累积死亡率显著降低。结果表明,饲料中添加一定量的GSH能显著提高罗非鱼的抗氧化性能和抗亚硝基氮应激能力。
A feeding trial was conducted to investigate the effects of dietary glutathione （GSH） on the antioxidant capabili-ty and resistance to nitrite exposure of GIFT Oreochromis niloticus. The fish, with an initial weight of （3.27 ± 0. 04） g, was fed a basal diet and five diets added with 80, 160, 240, 320, 400 mg/kg GSH respectively, named G0, G80, G160, G240, G320 and G400. After 49 days＇ feeding, it was found that glutathione S-transferase in serum of G320 and liver of G160 - G240, glutathione reductase in serum of G160 - G240 and liver of G320, superoxide dismutase activities in liver of G240, total antioxidant capacity in serum of G240 - G400 and liver of G240, anti-superoxide activities in serum of G320, were significantly higher than those of G0. Malondialdehyde content in liver of G80 - G320 significantly de-creased in comparison with GO. Accumulative mortality rate under nitrite exposure for 96 h in （;320 was significantly lower than GO. In conclusion, dietary GSH could improve the antioxidant capability and resistance to nitrite exposure of GIFT O. niloticus.
出处
《淡水渔业》
CSCD
北大核心
2013年第1期59-62,68,共5页
Freshwater Fisheries
基金
广东省基金团队项目(10351064001000000)
应用基础重点项目(11C14070782)
引进国外技术
管理人才项目(20114400003)
