摘要
为了通过诱变筛选获得豆豉溶栓酶高酶活菌株。研究以豆豉溶栓酶产生菌株Bacillus subtilis LD-8547为出发菌株,分别通过紫外线诱变和硫酸二乙酯的复合诱变,根据奶粉平板和血粉平板上菌落透明圈的大小进行初筛和复筛。并采用四肽底物测定法进行了溶栓酶的酶活力测定。结果表明,通过实验获得了产豆豉溶栓酶酶活力达18 228 U/mL的LD-8547-25菌株,比诱变出发菌株的酶活力提高了107%。为豆豉溶栓酶高酶活菌株的诱变筛选提供了有益的试验数据。
In order to obtain the strain producing Douchi fibrinolytic enzyme with higher activity.Douchi fibrinolytic enzyme producing strain Bacillus subtilis LD-8547 was treated by ultraviolet radiation and DES.After screening,a strain with high fibrinolytic enzyme activity was obtained.After five generations of the mutant,its ability to produce the enzyme was still stable.And the catalytic activity of the mutation was 18 228 U/mL approximately all the time,1.07 times higher than that of the wild strain.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第6期1251-1255,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省科学技术厅自然科学基金资助项目(2011J05049)
福建省教育厅科技计划项目(JA10101)
关键词
溶栓酶
紫外诱变
硫酸二乙酯
诱变育种
fibrinolytic enzyme
ultraviolet mutation
ethyl sulfate mutation
mutation breeding
