摘要
应用多重PCR同时扩增 16SrRNA和cagA基因来检测幽门螺杆菌感染及其分型。PCR产物经DNA序列测定证实为特异性扩增 ,敏感度为 10 2 CFU/ml。 48株Hp菌株中 ,Ⅰ型菌株 (cagA + ) 2 6株 (5 4 2 % ) ;5 5 0份胃粘液标本 ,Hp阳性 (16SrRNA基因 + ) 2 6 1份 (4 7 5 % ) ,其中Ⅰ型Hp 139份 (5 3 3% )。
A multiplex PCR assay with simultaneous amplification of 16 SrRNA and cagA gene was developed for detection and typing of Helicobacter pylori(Hp).PCR products were confirmed to be special amplification by DNA sequence.The sensitivity was 10 2 CFU/ml.Among 48 Hp strains,26(54 2%)were type I Hp strains (cagA+).Of 550 gastric secretion samples,261(47 5%)were Hp positive(16 SrRNA gene+)and 139(53 3%)were type I.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2000年第3期148-150,共3页
Chinese Journal of Clinical Laboratory Science
