摘要
与一般植物叶片相比 ,果树叶片因富含多酚和多糖类物质 ,制备高分子量DNA比较困难。我们建立了一个从果树叶片制备高分子量DNA的有效程序 ,按照该方法制备的五种果树HMWDNA ,无叶绿体和线粒体DNA污染 ,无大量的非特异小片段的混杂 。
Compared with general plant leaves, fruit tree leaves are rich in polyphenols and polysaccharides. Therefore it is very difficult to prepare high molecular weight DNA from them. We developed an efficient high molecular weight (HMW) genomic DNA preparation procedure for fruit tree leaves. We successfully isolated HMW DNA from 5 species fruit tree leaves that represented the material background of fruit tree leaves. The HMW DNAs were with no chloroplast and mitochondrail DNA and little small molecular weight DNA contamination. It demonstrated that the HMW DNA was suitable for reproducible digestion by restriction enzymes, large genomic library construction and analysis of complex genome region.
出处
《高技术通讯》
EI
CAS
CSCD
2000年第4期1-3,共3页
Chinese High Technology Letters
基金
北京市自然科学基金项目!(5982004)
