摘要
目的探讨皮肤鳞状细胞癌(CSCC)中Livin蛋白的表达与CD3+T细胞浸润的情况,分析两者在CSCC发生、发展中的作用。方法采用免疫组织化学SP法检测40例CSCC组织中Livin蛋白表达及CD3+T细胞浸润情况,并以10例正常皮肤组织作对照,分析二者与CSCC临床病理特征的关系及其相关性。结果 CSCC组织中Livin蛋白的阳性表达率为70.0%(28/40),正常皮肤组织中Livin无表达。10例正常皮肤组织中CD3+T细胞数量较少,为8.70±2.25,40例CSCC中为21.87±5.21,差异有统计学意义(P=0.000)。Livin蛋白表达和CD3+T细胞的数量均与CSCC的病理分级和淋巴结转移有关(P<0.05),但二者之间无相关性(r=0.015,P=0.926)。结论 Livin蛋白可以作为判断CSCC恶性程度、评价预后的指标。CD3+T细胞亦可反映机体抗肿瘤特异性细胞免疫状态和生物学行为,并作为判断预后的重要指标。
Objective To study the expression of livin and CD3 ＋ T lymphocytes infiltration in cutaneous squamous cell carcinoma（CSCC） , and observe their possible role in the pathogenesis of CSCC. Methods The expression of livin and CD3 ＋ T lymphocytes infiltration were detected with immunohistoehemieal SP method in 40 CSCC tissues and 10 normal skin tissues. The relationships among livin, CD3 ＋ T lymphocytes and the clinic pathologic characters of CSCC were analyzed. Results The positive rate of livin in CSCC was 70.0% （28/40）, while normal skin tissue did not express livin. The number of CD3 ＋ T lymphocytes in normal skin samples was 8.70 ±2. 25, while it was 21.87±5.21 in CSCC examples,showing significant differences（P =0. 000）. The positive rate of livin and the number of CD3 ＋ T lymphoeytes were correlated with the tumor histological grade and lymph node metastasis （ P 〈 0. 05 ）, but they had no correlation（ r = 0. 015,P = 0. 926）. Conclusion Livin can be used for evaluating the malignant degree of CSCC and predicating the prognosis. The expression of CD3 ＋ T lymphocytes may be useful in estimating the local immune status and biological behaviors, and predicting the prognosis of CSCC.
出处
《临床肿瘤学杂志》
CAS
2012年第10期880-883,共4页
Chinese Clinical Oncology
基金
江苏省徐州市科技计划发展基金资助项目(XM09B102)
