摘要
[Objective]The aim was to survey relationship between acute canine distemper and parasitic enteritis from pathology.[Method]Twelve cases of acute canine distemper with diarrhea were researched as per immunohistochemistry,Haematoxylin Eosin,and PAS staining kit.[Result]Of the twelve diseased dogs(with diarrhea),six were detected caused by coccidium and two were detected by cryptosporidium.Coccidian protozoa is mainly in epithelial cells of jejunum and ileum,and some can be found in cut-off intestinal epithelial cells and in mucus formed by destroyed intesti-nal villus.The most common shapes of coccidian protozoa are trophozoite and schizont.The former is mainly within or among epithelial cells;nucle-us is in center and stained by hematoxylin;protoplasm is in"fined mesh"shape.The latter,round or oval,contains much glycogenosome in de-generated intestinal epithelial cells.On the other hand,cryptosporidium is mainly in striated borders of intestinal epithelial cells and intestinal gland cells,leading to destruction of villus and cut-off of cells.Through detection on monoclonal antibody of nucleocapsid proteins of anti-canine distemper virus,it was found that epithelial cells in intestinal mucosa,glandular cells in recesses,lymphocytes and macrophage infittrated in lamina propria and dendritic cells in aggregated nodule were all with positive reactions.[Conclusion]Parasitic diarrhea caused by acute canine distemper occurs when resistance of intestinal mucosa caused by canine distemper virus begins to decline.
基金
funded by Scientific Research Staring Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China
Foundation of Talents and Science Researching,Henan Institute of Scince and Technology
