摘要
目的 为初步探讨TPA用于喉癌治疗的可行性 ,对TPA分别诱发Hep 2和原代喉癌细胞的凋亡作用进行了对比观察。方法 应用琼脂糖凝胶电泳及FCM技术分别对两种细胞中的凋亡细胞进行定性、定量及细胞周期检测。结果 ① 1nMTPA作用 12h ,Hep 2和原代喉癌细胞的凋亡率分别是 11.8%和 10 .14 %(P >0 .0 5 ) ;10 0nMTPA作用 2 4h ,凋亡率达峰值 ,分别为 2 7.80 %和 2 5 %(P >0 .0 5 ) ,细胞周期阻滞于G1期 ,DNA琼脂糖凝胶电泳可检出梯状带。②TPA诱发两种细胞的凋亡率具有明显的正相关 (r =0 .9674,P <0 .0 1)。结论 TPA在体外对Hep 2和原代喉癌细胞均可诱发凋亡 。
? Objective To investage the possibility of clinical treatment of laryngeal carcinoma with TPA,we compared the results of apoptosis induced by TPA in Hep 2 and primary laryngeal squamous carcinoma cells (PLSCC).Methods Electrophoresis was used to identify apoptosis.Apoptotic rates and cell cycle were detected with FCM (flow cytometry).Results After 12 hours treatment with TPA,apoptotic rates of the two kinds of cells were 11.18% and 10.14%(P>0.05),respectively.At 24th hour of the treatment with 100nM TPA,the apoptotic rates reached the maximum values:for Hep 2 and PLSCC were 27.8%,25%(P>0.05),respectively.Cell cycle was inhibited in G 1 phase.DNA agarose gel electrophoresis showed ladder belts.The apoptotic rates of Hep 2 had significantly positive correlation with PLSCC(r=0.9674,P<0.01).Conclusions TPA can induce apoptosis of both Hep 2 and PLSCC in vitro.The apoptotic rates of two kinds of cells have a better positive correlation. 〔
出处
《中国肿瘤临床与康复》
2000年第3期16-18,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
喉肿瘤
细胞凋亡
HEP-2
TPA
primary laryngeal carcinoma
cell apoptosis
Hep-2
TPA
