摘要
[目的]寻找前列腺肿瘤组织与正常组织的差异表达蛋白。[方法]运用蛋白质组学的方法分析前列腺癌组织和正常组织蛋白表达谱的差异,通过双向电泳筛选差异蛋白,质谱鉴定。[结果]成功构建了前列腺组织的双向电泳图谱,并筛选出一个差异蛋白,该差异蛋白在17例(17/24,70.8%)前列腺癌组织中表达上调,仅在5例(5/24,20.8%)对照组中呈高表达,两者具有统计学差异(P=0.001)。经鉴定,该差异蛋白为促凋亡胱天蛋白酶衔接蛋白(PACAP)。[结论]促凋亡胱天蛋白酶衔接蛋白可以视作潜在的前列腺癌肿瘤标志物。
[ Purpose] To explore the differentially expressed protein between prostate cancer tissue and normal tissue. [Methods] Proteomic technology was applied to analyze protein expression profile difference between prostate cancer tissue and normal tissue. Two-dimensional gel elec- trophoresis was used to screen differentially expressed proteins,which was then identified by mass spectrometry. [Results ] Two-dimensional gel electrophoresis profiles of prostate tissues were es- tablished. One protein spot was screened out,which was over-expressed in 17 （17/24,70.8%） prostate cancer tissues but only in 5 （5/24,20.8%） normal prostate tissues,with significant diffe- rence between them （P=-0.001）. The protein spot was identified by mass spectrometry as proapop- totic caspase adaptor protein （PACAP）. [Conelusionl The proapoptotic caspase adaptor protein might be a potential biomarker for prostate cancer.
出处
《肿瘤学杂志》
CAS
2012年第8期572-574,共3页
Journal of Chinese Oncology
基金
延安大学附属医院创新基金(201102)
