摘要
目的:建立反相高效液相色谱法快速测定丹参中丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量。方法:采用Kinetex核-壳技术色谱柱(100 mm×4.6 mm,2.6μm),以乙腈-0.1%三氟乙酸水溶液为流动相,梯度洗脱,柱温30℃,检测波长为280 nm(丹酚酸B)和254 nm(丹参酮类)。结果:丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA5个成分浓度分别在3.40～850μg.mL-1(r=0.9998),0.96～240μg.mL-1(r=0.9999),2.04～255μg.mL-1(r=0.9995),0.27～68μg.mL-1(r=0.9998),1.12～210μg.mL-1(r=0.9997)范围内与峰面积呈现良好的线性;平均回收率(n=3)在97.32%～104.9%之间,RSD≤4.7%。结论:该方法快速、简便、灵敏,可用于丹参药材的质量控制。
Objective：To establish a RP-HPLC method for the fast simultaneous determination of salvianolic acid B,dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ,and tanshinone ⅡA in Radix et Rhizoma Salviae Miltiorrhizae samples.Methods：These five constituents were analyzed on a Kinetex core-shell technology high performance chromatography column（100 mm×4.6 mm,2.6 μm） by a gradient elution using 0.1% trifluoroacetic acid aqueous solution and acetonitrile as the mobile phase at 30 ℃ column temperature;the detection wavelength was set at 280 nm for salvianolic acid B and 254 nm for tanshinones.Results：The results showed good linearities in the range of 3.40-850 μg·mL-1（r=0.9998） for salvianolic acid B,0.96-240 μg·mL-1（r=0.9999） for dihydrotanshinone Ⅰ,2.04-255 μg·mL-1（r=0.9995） for cryptotanshinone,0.27-68 μg·mL-1（r=0.9998） for tanshinone Ⅰ,and 1.12-210 μg·mL-1（r=0.9997） for tanshinone ⅡA;the average recoveries of the five constituents ranged between 97.32% and 104.9%（RSD≤4.7%）.Conclusion：The method is rapid,simple and sensitive which can be used for the quality control of Radix et Rhizoma Salviae Miltiorrhizae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第8期1357-1361,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金项目(30973883)
关键词
丹参
丹酚酸B
丹参酮类
二氢丹参酮
高效液相色谱
多指标成分分析
快速测定
中药质量控制
Radix et Rhizoma Salviae Miltiorrhizae
salvianolic acid B
tanshinones
dihydrotanshinone
HPLC
multiple index constituents analysis
fast determination
quality control of traditional Chinese medicine
