摘要
用石油醚和乙酸乙酯分别对白背黄花稔Sida rhombifolia L.地上部分的乙醇提取物进行萃取,得不同萃取相;测定了乙醇提取物及不同萃取相对白纹伊蚊Aedes albopictus Skuse 4龄幼虫和福寿螺Ampullaria gigas Spix的毒杀活性。结果表明,乙醇提取物和乙酸乙酯相对白纹伊蚊4龄幼虫的杀虫活性明显,而石油醚相和乙醇提取物对福寿螺的杀螺活性较好。对乙酸乙酯相进一步分离得到11个化合物,经核磁共振氢谱(1H NMR)和碳谱(13C NMR)鉴定其分别为已知化合物:间-羟基苯甲酸(1)、棕榈酸1-甘油酯(2)、丁香酸(3)、没食子酸(4)、β-谷甾醇(5)、豆甾醇(6)、东莨菪内酯(7)、山奈酚(8)、5-羟基-7,4'-二甲氧基二氢黄酮(9)、3-O-β-D-葡萄糖-(6-O-对羟基反式香豆酰基)-山奈素苷(10)和β-谷甾醇-3-O-葡萄糖苷(11)。其中化合物2对白纹伊蚊4龄幼虫具有较好的杀虫活性,24 h和48 h的LC50值分别为28.66和13.50μg/mL。
The ethanol extract of the aerial part of Sida rhombifolia L.was extracted with petroleum ether and ethyl acetate to give the petroleum ether extract,ethyl acetate extract and water phase.Toxic activity of the ethanol extract and three crude extracts against the 4th-instars larva of Aedes albopictus Skuse and Ampullaria gigas Spix was tested.It showed that the ethanol extract and ethyl acetate extract had obvious insecticidal activity against the 4th-instars larva of A.albopictus,while petroleum ether extract and the ethanol extract showed higher molluscicidal activity against A.gigas.Eleven known compounds were isolated from the ethyl acetate extract through further phytochemical investigation,their structures were identified as m-hydroxybenzoic acid（1）,glyceroyl monopalmitate（2）,syringic acid（3）,gallic acid（4）,β-sitosterol（5）,stigmasterol（6）,scopoletin（7）,kaempferol（8）,5-hydroxy-7,4′-dimethoxyflavanone（9）,tiliroside（10） and β-sitosterol-3-O-glucoside（11）,respectively,by 1H NMR and 13C NMR.Compound 2 exhibited potent insecticidal activity against the 4th-instars larva of A.albopictus,with LC50 value of 28.66 μg/mL and 13.50 μg/mL after treatment in 24 h and 48 h,respectively.
出处
《农药学学报》
CAS
CSCD
北大核心
2012年第4期377-382,共6页
Chinese Journal of Pesticide Science
基金
国家公益性行业(农业)科研专项基金资助项目(200903052)
关键词
锦葵科
白背黄花稔
生物活性
化学成分
Malvaceae
Sida rhombifolia L.
bioactivity
chemical constituent
