摘要
【目的】明确中国北方地区菜豆普通细菌性疫病病原菌。【方法】应用菌落特征、油菜黄单胞菌菜豆变种诊断试剂检测、致病性测定、16S rDNA和16S-23S rDNA ITS序列分析、特异PCR检测、脂肪酸分析及生理生化反应特征对病原菌分离物进行鉴定。【结果】从病害样品和菜豆种子样品中均分离到类似黄单胞杆菌的细菌分离物,选取25个代表分离物进行致病性测定,有24个分离物在菜豆品种"英国红"上导致典型菜豆普通细菌性疫病症状。结合16S rDNA及16S-23S rDNA ITS序列比对分析、特异性PCR检测及生理生化反应的结果,24个分离物中7株被鉴定为地毯草黄单胞菌菜豆变种,17株为褐色黄单胞菌褐色亚种。【结论】中国北方地区发生的菜豆普通细菌性疫病由地毯草黄单胞菌菜豆变种或(和)褐色黄单胞菌褐色亚种引起;褐色黄单胞菌褐色亚种在中国属首次报道。
[ Objective] The objective of this study is to identify the pathogens that cause common bacterial blight on common bean in north China. [Method] The causal agents were identified by colony characterization, detection using Xanthomonas campestris pv. phaseoli diagonsis kit （ADGEN EXPRESSTM）, pathogenicity test, sequencing 16 rDNA and 16S-23S rDNA intergenic spacer （ITS） regions, PCR detection using specific primers for X. axonopodis pv. phaseoli and X. fuscans subsp, fuscans, fatty acid methyl esters （FAME） analysis and biochemical characterization tests. [ Result ] Bacterial isolates of Xanthmonads-like were isolated from all diseased plant and seed samples. Twenty-five representative isolates were selected for pathogenicity test, and 24 tested isolates casused typical symptoms of common bacterial blight on the inoculated plants of common bean cutivar ＇Yingguohong＇. Based on pathenogenicity test, and combined with the results of diagonsis kit and specific PCR detection, biochemical and molecular characterization, 7 isoaltes were identifed as X. axonopodis pv. phaseoli, and 17 isolates were identified as X. fuscans subsp, fuscans. [ Conclusion ] The common bacterial blight on common bean in north China was incited by X. axonopodis pv. phaseoli or/and X. fuscans subsp.fuscans. It was the first report ofX.fuscans subsp.fuscans in China.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第13期2618-2627,共10页
Scientia Agricultura Sinica
基金
国家现代农业产业技术体系建设专项资金(CARS-09)
作物种质资源保护项目(NB2010-2130135-25-14)
