摘要
目的建立乌参醒脑滴丸(人参、首乌,银杏叶提取物)中人参皂苷Rg1、Re、Rb1和Rd的HPLC测定方法。方法采用Diamonsil-C18色谱柱(150 mm×4.6 mm,5.0μm),柱温30℃;流动相为乙腈和水进行梯度洗脱,体积流量为1.0mL/min;检测波长为203 nm。结果人参皂苷Rg1、Re、Rb1和Rd分别在11.68～58.4μg/mL、15.30～153.0μg/mL、13.25～132.5μg/mL、7.65～76.5μg/mL质量浓度范围内与峰面积呈良好的线性关系,4种皂苷类成分的平均回收率为97.5%(RSD为1.56%)、100.3%(RSD为2.13%)、97.6%(RSD为1.98%)、98.6%(RSD为1.53%)。结论该法灵敏、简便、准确,可用于乌参醒脑滴丸的质量控制。
AIM To establish an HPLC method for determining the contents of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd in Wushen Xingnao Dropping Pill（Ginseng Radix et Rhizoma,Polygoni multiflori Radix,Gingko leaves extract）.METHODS Chromatography was performed on a Diamonsil-C18（150 mm×4.6 mm,5.0 μm） at 30 ℃.The mobile phase was composed of acetonitrile and water in gradient mode with a flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm.RESULTS The calibration curves of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd were linear in the ranges of 11.68-58.4 μg/mL,15.30-153.0 μg/mL,13.25-132.5 μg/mL,7.65-76.5 μg/mL,respectively.The average recoveries of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,ginsenoside Rd were 97.5%,100.3%,97.6%,98.6%,respectively.And RSDs were 1.56%（n=6）,2.13%（n=6）,1.98%（n=6）,1.53%（n=6）,respectively.CONCLUSION The method can be used for quantitative determination of the preparation.
出处
《中成药》
CAS
CSCD
北大核心
2012年第7期1280-1283,共4页
Chinese Traditional Patent Medicine
基金
广东药学院2007年度人才引进基金资助项目(2007ZY103)
广州市科技计划资助项目(11C32130738)
