摘要
目的探讨丹参水溶性单体成分原儿茶醛对胶原酶诱导APOE-/-小鼠内皮损伤的保护作用。方法采用尾静脉注射胶原酶II合并肝素法建立内皮损伤模型;采用生理盐水同步处理作为阴性对照组,实验组造模同时同步腹腔注射原儿茶醛。采用扫描电镜观察动脉内膜完整性和内皮细胞的形态结构;采用流式细胞胞内外染色法检测小鼠脾脏淋巴细胞对TNF-α、IL-17等细胞因子的表达能力。结果电镜结果显示:正常小鼠和对照组小鼠内膜和内皮细胞间连接完整,偶有内皮细胞脱落;而内皮损伤模型组小鼠主动脉内膜不完整,内皮细胞明显脱落,聚集成团,条索部分紊乱,细胞间连接丢失,造成空洞或"蜂窝"状内膜;PCAD实验组血管内膜基本规整,内皮细胞偶有脱落,无明显附着物。流式细胞术结果显示:随着内皮损伤和细胞的脱落,脾脏淋巴细胞分泌炎症因子IL-17、TNF-α水平较对照组小鼠明显升高,而PCAD实验组与模型对照组相比可下调炎症因子的阳性比例(P<0.05)。结论丹参水溶性成分PCAD可明显保护动脉粥样硬化早期的内皮损伤,下调炎性因子的释放。
Objective To investigate the protective effect of Protocatechualdehyde of the water-soluble ingredients of Salvia miltiorrhiza on endothelial injury induced by collagenase in APOE-/-mice.Methods Endothelial injury model was established by intravenous injection of collagenase II with heparin;mice of negative control group was synchronized with physiological saline,the experimental group while simultaneously modeling the intraperitoneal injection of Protocatechualdehyde.Using scanning electron microscope artery intimal integrity and endothelial cell morphology were oberved.And using flow cytometry intracellular and extracellular staining the cytokine expression of TNF-α and IL-17 was measured in splenic lymphocytes of mouse.Results The electron microscopy results showed that the connection between the mouse intima and endothelial cell integrity was normal in normal mice and control group,and occasional endothelial cell shedding occurred.In endothelial injury model mice,aortic intima was incomplete,and endothelial cells was significantly exfoliated and gathered into cluster.The cord was partly disturbed,and the connection among cells was lost resulting in the the empty or ＂honeycomb＂-like intima.In the PCAD experimental group,it were regular intima,occasional endothelial cell shedding,and no significant attachments.Flow cytometry results showed that endothelial cell injury and cell detachment were present,the secretion of inflammatory cytokines such as IL-17 and TNF-α in splenic lymphocytes was significantly increased compared with the control group of mice,while the PCAD experimental group compared with control group compared inflammatory factors were down-regulated（P〈0.05）.Conclusion PCAD,the water-soluble ingredients of Salvia miltiorrhiza can attenuates early endothelial injury in atherosclerosis and decrease the release of inflammatory factors.
出处
《济宁医学院学报》
2012年第3期173-176,共4页
Journal of Jining Medical University
