摘要
目的总结在美国国立卫生研究院临床中心建立大鼠腹腔内心脏移植模型的经验，探讨手术成功的主要影响因素。方法以80只DarkAgouti大鼠为供体，70只BrownNorway大鼠为受体，制作大鼠心脏移植模型。以连续20例手术死亡率低于15％且无明显合并症为手术合格标准。结果为达到合格标准，共施行了10例大鼠供体手术，70例大鼠移植手术。最后20例移植手术成功率为95％，平均手术时间为55min，供心平均缺血时间为35min。结论精准的显微外科技术，加上标准化手术训练和严格的动物管理，是保证手术成功率的关键因素。
Objective To review the experiences and key factors of establishing an abdominal cardiac transplantation model in rats at National Institutes of Health Clinical Center. Methods Dark Agouti rats served as donor while Brown Norway rats as recipient. Cardiac transplantations were performed with a modified version of heterotopic cardiac transplantation. Proficiency was defined as 20 consecutive operations with 〈 15% mortality and no significant major co-morbidities. Results We performed 10 donor harvest- alone operations and 70 transplantations to reach the proficiency level. The survival rate of the last 20 consecutive surgeries was 95%. The mean total operative duration was 55 min and mean ischemic time of donor hearts 35 rain. Conclusion To ensure a consistently high operative success rate, a surgeon must not only be proficient in micro-surgical skills, but also closely follow the standardized training procedures and strictly observe the animal care and use guidelines.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第24期1715-1718,共4页
National Medical Journal of China
基金
上海市科学技术委员会浦江人才计划（10PJ1403600）
NIH Clinical Center Intramural Research Program （ CCM07-03 .CCM10-05 ）
关键词
心脏移植
模型
动物
大鼠
Heart transplantation
Models, animal
Rats
