摘要
目的:观察资木瓜总苷(TSCS)对佐剂性关节炎(AA)大鼠滑膜组织病理改变、滑膜肥大细胞脱颗粒及类胰蛋白酶表达的影响,探讨肥大细胞功能与TSCS治疗大鼠AA的关系。方法:雄性Wistar大鼠随机分为正常对照组、模型组和TSCS组(200 mg.kg-1),每组15只,致炎后第3天开始ig给药,1次/d,模型组和正常对照组给予等容积生理盐水,持续21 d。除正常对照组外,其余大鼠足部注射弗氏完全佐剂诱导建立AA模型,检测大鼠体质量和足跖体积变化,HE染色评价滑膜组织病理改变,TB染色观察滑膜肥大细胞数量及其脱颗粒情况,免疫组化法检测滑膜组织中类胰蛋白酶表达情况。结果:TSCS能明显增加AA大鼠体质量,减轻足跖肿胀度,显著减轻滑膜组织炎性细胞浸润、滑膜细胞增生和滑膜纤维组织增生,有效抑制滑膜肥大细胞数量和脱颗粒率,显著抑制滑膜组织类胰蛋白酶表达。相关分析显示,滑膜肥大细胞数量及脱颗粒率均与滑膜组织病理改变总评分呈明显的正相关。结论:TSCS具有治疗大鼠AA和调节滑膜肥大细胞功能的作用,由于两者存在明显的正相关性,因此TSCS可能通过抑制滑膜肥大细胞功能,对AA大鼠起治疗作用。
Objective： To observe the effects of total saponins of Chaenomeles speciosa （TSCS） on synovial pathology, synovial mast cell degranulation and tryptase expression and to investigate the relationship between the functions of mast cells and effects of TSCS on adjuvant arthritis （AA） in rats. Method： Male Wistar rats were randomly divided into normal control group, model group and TSCS group （200 mg· kg-1） , n = 15 in each group. AA was induced by injection of 0. 1 mL Freund＇s complete adjuvant in left hind limb footpad. Normal control group and model group received saline treatment, while rats in the TSCS group were treated once a day for 21 days. The body weight and paw volume of the rats were measured every six days. Synovial tissues of the right hind ankles were sampled and stained with HE for observing synovial pathology to evaluate the effects of TSCS on AA, then stained with TB for observing the number and degranulation ratio of synovial mast cells and finally detected by immunohistochemical staining method to investigate the expression of tryptase in synovium. Result： TSCS increased significantly the body weight of AA rats, while decreased obviously the paw volume. TSCS significantly inhibited inflammatory cell infiltration, synovial cell hyperplasia, and synovial fibroplasia in synovium of AA rats. TSCS could significantly diminish the numbers of total and degranulated mast cells in AA rats, TSCS decreased the expression of tryptase in synovium. Analyzed by Spearman＇s bivariate correlation, the number of mast cells and degranulation ratio of mast ceils were positively correlated with the pathological scores. Conclusion： TSCS can improve pathological condition of inflammatory synovium in AA rats by inhibiting the function of synovial mast cells, which may play an important underlying role in the immunoregulation of TSCS on AA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期173-178,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖北省教育厅项目(B20101201)
宜昌市科技局项目(2010A01301-04)
三峡大学科研基金项目(0620080702)
宜昌市医疗卫生科技项目(A06425)
关键词
资木瓜总苷
佐剂性关节炎
肥大细胞
脱颗粒
类胰蛋白酶
total saponins of Chaenomeles speciosa
adjuvant arthritis
mast ceils
degranulation
tryptase
