摘要
目的 :探讨杀菌 /通透性增强蛋白 (BPI)对腹腔感染脓毒症大鼠的治疗作用及其机制。方法 :腹腔感染脓毒症模型采用盲肠结扎穿孔法 ,分别在盲肠结扎穿孔后即刻和 12h腹腔内注射BPI各 2 5mg/kg或等容量生理盐水 ,血浆内毒素采用鲎试验显色定量测定法。结果 :(1)BPI治疗组动物存活时间明显长于生理盐水组 ;(2 )BPI治疗组动物平均动脉压 ,左室收缩压 ,心室等容收缩压 ,左室压力最大上升和下降速度等虽有一定下降 ,但均显著高于生理盐水组 ;(3)BPI组动物血浆GPT、尿素氮水平有一定升高 ,但明显低于生理盐水组 ;(4 )BPI组血浆内毒素水平无明显变化 ,而生理盐水组明显升高 ,两组间有显著性差异。结论 :BPI对腹腔感染脓毒症大鼠有明显的治疗作用 ,其机制与其拮抗内毒素活性有关。
AIM:To investigate the protective effect of bactericidal/permeability-increasing protein (BPI) on sepsis induced by intra-abdominal infection in rats and its mechanism.METHODS:Intra-abdominal infection induced sepsis was reproduced by cecal ligation and puncture (CLP). BPI or equal volume of physiological saline was intra-abdominally given immediately after CLP and 12 hours after CLP respectively (2.5 mg/kg of BPI each time). Plasma endotoxin levels were determined with limulus amebocyte chromogenic assay.RESULTS:(1)The survival time in BPI group was significantly higher than in physiological saline (PS) group. (2)The values of MAP, LVSP, IP, d p /d t max and -d p /d t max in BPI group, although decreasing ,were markedly higher than those in PS group. (3) Plasma glutamate-pyruvate transaminase and urea nitrogen levels in BPI group, though increasing, were significantly lower than those in PS group.(4) There was no significant change of plasma endotoxin levels in BPI group, while plasma endotoxin levels were markedly increased in PS group. There was significantly different between two groups. CONCLUSIONS:BPI has an obvious protective effect on intra-abdominal infection induced sepsis, which might be related to its antagonism against endotoxin.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第2期120-122,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目! (39470 6 78)
