摘要
目的:探讨雷帕霉素对前列腺癌细胞22RV1增殖及对S6 Kinase 1(S6K1)活性的影响。方法:用不同浓度(0、50、100、200、400 nmol/L)雷帕霉素作用于体外培养的前列腺癌细胞22RV1,MTT法检测细胞生长抑制率;应用液闪激酶活性测定法检测不同浓度雷帕霉素对S6K1活性的影响。结果:雷帕霉素能显著抑制22RV1细胞的生长,呈现明显的量-效关系,随着雷帕霉素剂量的增加,细胞生长抑制率逐渐升高,400 nmol/L的雷帕霉素对22RV1细胞的抑制率最高(P<0.01);同时雷帕霉素还能使S6K1蛋白活性下降,随剂量增高降低越明显,400 nmol/L的雷帕霉素使S6K1蛋白活性下降最明显(P<0.01)。结论:雷帕霉素可通过调控mammal Target ofrapamycin(mTOR)下游蛋白S6K1表达来有效地抑制前列腺癌细胞22RV1的增殖。
Objective： To explore the effects of rapamycin on the proliferation of prostate cancer cell line 22RV1 and the activity of S6K1.Methods： Prostate cancer 22RV1 cells cultured in vitro were treated with rapamycin at the concentrations of 0,50,100,200 and 400 nmol/L.The inhibition rate of the cells＇ proliferation was detected by MTT,and the activity of S6K1 was determined by liquid scintillation counting.Results： Rapamycin significantly inhibited the proliferation of the prostate cancer 22RV1 cells and the activity of S6K1 in a dose-dependent manner,most obviously at 400 nmol/L（P0.01）.Conclusion： Rapamycin can effectively suppress the proliferation of prostate cancer 22RV1 cells by regulating the expression of S6K1,the downstream protein of mammalian target of rapamycin（mTOR）.
出处
《中华男科学杂志》
CAS
CSCD
2012年第4期327-330,共4页
National Journal of Andrology
