摘要
目的:观察丹蒌片对异丙肾上腺素(isoproterenol,Iso)所致急性心肌梗死(acute myocardial infarction,AMI)大鼠心肌梗死面积和心肌原癌基因Bcl-2(B-cell lymphoma leulemia-2,Bcl-2)、半胱氨酸天门冬氨酸蛋白酶-3(cysteinyl aspartates pecific proteinase-3,Caspase-3)表达的影响。方法:雄性Wistar大鼠40只随机分为4组:空白组(生理盐水10 mL·kg-1);模型组(生理盐水10 mL·kg-1);丹蒌高剂量组(900 mg·kg-1);丹蒌低剂量组(450 mg·kg-1),各组10只,连续ig 14 d。14 d后除空白组以外,其他各组一次性sc Iso 15 mg·kg-1制备急性心肌梗死模型。造模后24 h观测心肌病理形态学、梗死面积、心肌Bcl-2和Caspase-3蛋白表达水平。结果:与模型组比较,丹蒌片900 mg·kg-1可明显减轻缺血心肌病理损害程度,缩小心肌梗死面积[2组依次为(2.185±0.637)%及(1.224±0.365)%,P<0.01],上调缺血心肌Bcl-2积分吸光度(IA):(27.32±7.00及42.41±8.13,P<0.01)蛋白表达,下调心肌Caspase-3积分吸光度(IA):(38.40±6.33及26.26±6.79,P<0.01)蛋白表达。结论:丹蒌片通过上调心肌Bcl-2蛋白表达,下调Caspase-3蛋白表达,减轻心肌细胞凋亡,减少心肌梗死面积,保护心肌缺血损伤。
Objective：To study the effect of Danlou tablet on myocardial Infarct size and expression of Bcl-2 and Caspase-3 protein in rats with acute isoproterenol-induced myocardial infarction.Method：Forty male Wistar rats were randomly divided into 4 groups： Control group（normal saline 10 mL·kg-1·d-1,n=10）,model group（normal saline 10 mL·kg-1·d-1,n=10）,Danlou high dose group（900 mg·kg-1·d-1,n=10）,Danlou low dose group（450 mg·kg-1·d-1,n=10）.Each group was ig given for 14 d.Except for control group,the acute myocardial infarction model was induced by subcutaneous injection of Iso 15 mg·kg-1 in 14 d afte administration.The pathological changes in myocardial,myocardial infarct size and expression of myocardial Bcl-2,Caspase-3 protein were observed in 24 h after injection of Iso.Result： Compared with model group,Danlou tablet could significantly reduce the extent of ischemic myocardial injury,reduced myocardial infarct size（2.185±0.637）% vs（1.224±0.365）%,P0.01）,Bcl-2 IA：（27.32 ± 7.00） vs（42.41 ± 8.13）,（P0.01） protein expression was up-regulated,and Caspase-3 IA：（38.40±6.33） vs（26.26±6.79）,（P0.01）protein expression was reduced,conclusion Danlou tablet can up-regulate Bcl-2 protein expression and reduce protein expression of Caspase-3,which may be related to protection against myocardial ischemic injury.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期216-219,共4页
Chinese Journal of Experimental Traditional Medical Formulae
