摘要
目的:观察石藤胶囊对大鼠佐剂性关节炎(adjuvant arthritis,AA)血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)含量的影响。方法:采用弗氏完全佐剂诱导的大鼠AA模型。选用Wistar大鼠,造模后第19天,将出现多发性关节炎症状的大鼠随机选取24只,并随机分为模型组、石藤胶囊组、雷公藤组,每组8只。注射生理盐水大鼠8只作为正常对照组。各组按每日10 mL·kg-1连续灌胃给药或给等体积生理盐水,持续21 d。其中石藤胶囊组每日灌服石藤胶囊0.75 g.kg-1,雷公藤组每日灌服雷公藤多苷片6 mg·kg-1,模型组和正常组灌服等体积的生理盐水。放射免疫法测定大鼠血清TNF-α,IL-1β,同时观察大鼠足爪肿胀抑制率、关节炎指数及关节病理积分的变化。结果:石藤胶囊可以明显降低AA大鼠血清TNF-α[模型组为(1.25±0.22)μg·L-1,石藤胶囊组为(0.88±0.11)μg·L-1,两组对比P<0.01]和IL-1β水平[模型组为(0.28±0.05)μg·L-1,石藤胶囊组为(0.20±0.03)μg·L-1,两组对比P<0.01)],降低AA大鼠的足爪肿胀度,提高对关节肿胀的抑制率。结论:石藤胶囊能够通过降低血清TNF-α和IL-1β水平对AA发挥治疗作用。
Objective：To observe the effect of Shiteng capsules on the content of tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in blood serum of rats with adjuvant arthritis（AA）.Method： Thirty-two Wistar rats were randomly divided into 4 groups as follows： control group,model group,shiteng capsules group,tripterygium wilfordii group.The model was induced by Freund＇s complete adjuvant.The TNF-α and IL-1β level in blood serum was detected by radioimmunity,at the same time,the inhibition ratio of paw edema,arthritis index and pathological score was observed.Result： Shiteng capsules cauld decrease TNF-α and IL-1β level in rats with AA obviously（compared with model group,P0.01）,and increse the inhibition ratio of paw edema.Conclusion： Shiteng capsules can play therapeutical effect to AA by redusing the levevl of serum TNF-α and IL-1β.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期195-198,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南中医学院博士科研基金项目(BSJJ2009-18)
