摘要
目的研究人乳头状瘤病毒 HPV-E6和 p53在食管鳞癌中的表达,以探讨它们在食管鳞癌中发生发展的生物学意义.方法应用 LSAB 免疫组化方法对16例正常食管粘膜,18例慢性炎症,22例不典型增生以及60例食管鳞癌组织进行HPV-E6和 P53蛋白表达的研究.结果在正常食管粘膜、慢性炎症、不典型增生及食管鳞癌中HPV-E6蛋白阳性率分别为37%,39%,50%及73%,P53蛋白阳性率在上述四组中则为0%,6%,41%及63%.四组比较,HPV-E6和 P53蛋白表达均有显著差别(P<0.05).在60例食管鳞癌Ⅰ,Ⅱ,Ⅲ级分组中,HPV-E6阳性率分别为59%,78%和85%,p53阳性率则分别为45%,67%,80%.三个分级组比较,HPV-E6和 P53蛋白表达均有显著差别(P<0.05).结论 HPV-E6及 p53与食管鳞癌的发生发展有着一定相关性,且 HPV-E6及 p53共同作用很可能更为食管鳞癌发生发展的重要因素.
AIM To investigate the mechanisms of esophageal carcinogenesis by HPV-E6 infection in esophagus as well as expression of P53 protein. METHODS HPV-E6 and P53 protein was examined by immunohistochemical method in 116 paraffin-embedded cases of different esophageal tissues. RESULTS HPV-E6 infection rate in normal mucosa, inflammatory mucosa,moderate or severe dysplasia and esophageal carcinoma tissues were 37%,39%.50% and 73%,at the same time,nuclear P53 protein positive were 0%,6%,41% and 63%.Besides,the expression of HPV- E6 and P53 protein in 60 carcinomas is closely associated with the pathohistological grading and HPV-E6 infection with the positive expression of p53 in carcinoma tissues was significantly higher than HPV-E6 negative with the positive expression of p53 statistically. CONCLUSION HPV-E6 infection and p53 are intimately linked to esophageal carcinoma.The cooperation of HPV- E6 and p53 may play an important role in esophageal carcinogenesis.
出处
《世界华人消化杂志》
CAS
2000年第5期494-496,共3页
World Chinese Journal of Digestology
