摘要
目的:优选纤维素酶提取无患子中总皂苷的最佳工艺。方法:运用正交设计实验方法步骤,以加入酶量、溶液pH值、酶提温度以及酶提时间作为4大影响提取率大小的因素,确定最佳的提取工艺。结果:最佳纤维素酶提取工艺:加入酶量为0.015%,pH值为4,50℃水浴酶提取2 h,测得平均提取率为38.98%。结论:纤维素酶提取法操作简单,稳定,提取率高。
Objective： To optimize the best cellulase extraction technology of Sapindus conaponin, method： Using orthogonal design experimental method of procedure, discussed the Influence of the amount of cellulase added, solution PH value, temperature and extraction time, to determine the optimal extraction process. Result： Optimum extracting condition： Adding cellulase to 0.15%, pH=4, 50 ℃ water bath extraction 2 h, measured the average extraction rate of 38.98%. Conclusion： This optimized process is simple, stability, high extraction efficiency.
出处
《中医药导报》
2012年第3期73-74,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
无患子
总皂苷
纤维素酶
正交设计
Supindus
Saponin
Orthogonal experiment
Cellulase extraction
