摘要
目的：探讨羟基红花黄色素A对鼠骨折端主要细胞（成骨细胞、内皮细胞、巨噬细胞）Toll样受体4（TLR4）表达的影响。方法SD大鼠股骨骨折对接后14天骨折端的骨痂，进行成骨细胞、内皮细胞的原代培养。实验分4组，即对照组、骨康泰灵组、羟基红花黄色素A高、低量组。应用RT—PCR及Western-blot方法检测TLR4 mRNA表达及蛋白含量。结果羟基红花黄色素A高剂量组成骨细胞、内皮细胞）、巨噬细胞TLR4 mRNA和蛋白含量低于对照组（P〈0．05）。结论：羟基红花黄色素A能降低成骨细胞、内皮细胞、巨噬细胞TLR4 mRNA和蛋白表达。
Objective To study the effects OF Safflomin A on the expression of TLR4 in callus osteoblasts,endothelial cells（EC）, macrophages （MC）. Methods Normal osteoblasts were obtained for primary cultured of callus .The cells were divided into control group, Safflomin A high dose group and Safflomin A low dose group. Total mRNA and protein content of each group were analyzed by RT-PCR and Western blot, respectively. Results The expression of TLR4 mRNA and protein content of osteoblasts,endothelial cells,macrophages Safflomin A high does group were significantly lower than in control group （ P 〈 0.05 ）.Conclusion Safflomin A could down-regulaate the the expression of TLR4 mRNA callus osteoblasts, endothelial cells, macrophages.
