摘要
目的建立测定五加参颗粒中紫丁香苷含量的高效液相色谱法。方法采用Agilent C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.3%甲酸溶液(体积比12∶88),流速为1.0 mL/min,检测波长为265 nm,柱温为30℃。结果紫丁香苷进样量在0.041 04～0.451 4μg(r=0.999 9)范围内与峰面积积分值线性关系良好,平均加样回收率为101.50%,RSD=1.96%(n=6)。结论高效液相色谱法灵敏度高、操作简便、准确可靠,可用于五加参颗粒中紫丁香苷的含量测定。
Abstract：Objective To develop a HPLC method for the determination of syringin in Wujiasheng Granules. Methods The Agilent C18 column（250 mm×4.6 mm,5 μm）was used with the mobile phase of acetonitrile-0.3% formic acid （12：88） at the flow rate of 1.0 mL/min. The detection wavelength was 265 nm and the temperature was 30 ℃. Results The good linear relation of syringin was in the injection volume of 0. 041 04-0. 451 4 μg（r = 0. 999 9）. The average recovery rate was 101.50%, RSD = 1.96% （n = 6）. Conclusion The method is sensitive,convenient and accurate,which can be used to determine the content of syringin in Wujiasheng Granules.
出处
《中国药业》
CAS
2012年第4期39-40,共2页
China Pharmaceuticals
